Transcript: Human NM_001205138.3

Homo sapiens leucine rich transmembrane and O-methyltransferase domain containing (LRTOMT), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
LRTOMT (220074)
Length:
2521
CDS:
612..1136

Additional Resources:

NCBI RefSeq record:
NM_001205138.3
NBCI Gene record:
LRTOMT (220074)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001205138.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130029 GAGAAAGGGTATAGGCAATAT pLKO.1 981 CDS 100% 13.200 18.480 N LRTOMT n/a
2 TRCN0000130826 GAAGAGAAAGGGTATAGGCAA pLKO.1 978 CDS 100% 2.640 3.696 N LRTOMT n/a
3 TRCN0000347011 CCATTGACCCTGTCCTAACAA pLKO_005 835 CDS 100% 5.625 4.500 N Lrrc51 n/a
4 TRCN0000148621 CCCTGTCCTAACAACTTTCTT pLKO.1 842 CDS 100% 5.625 4.500 N LRTOMT n/a
5 TRCN0000432190 ACAGCATGGTTTGACAATAAA pLKO_005 1177 3UTR 100% 15.000 10.500 N LRTOMT n/a
6 TRCN0000146854 CAATGATCTGAGAGACTTCAA pLKO.1 740 CDS 100% 4.950 3.465 N LRTOMT n/a
7 TRCN0000131103 GCATGAACATCAAGCCCAAGA pLKO.1 1087 CDS 100% 4.050 2.835 N LRTOMT n/a
8 TRCN0000146985 CCTAACAACTTTCTTCAACCT pLKO.1 848 CDS 100% 2.640 1.848 N LRTOMT n/a
9 TRCN0000150088 CCTTGTAGAGATGTTCTCTAA pLKO.1 1235 3UTR 100% 0.495 0.347 N LRTOMT n/a
10 TRCN0000424730 GACCAAGCAGAATACACTTTG pLKO_005 1115 CDS 100% 10.800 6.480 N LRTOMT n/a
11 TRCN0000072628 CCTCCCAAAGTGCTAGGATTA pLKO.1 2108 3UTR 100% 10.800 5.400 Y MRPS16 n/a
12 TRCN0000140657 CCTCCCAAAGTGCTAGGATAA pLKO.1 2108 3UTR 100% 10.800 5.400 Y CD3EAP n/a
13 TRCN0000141125 CAGGTTCAAGAGATTCTCCTA pLKO.1 1381 3UTR 100% 2.640 1.320 Y SYNE4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001205138.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05241 pDONR223 100% 89.7% 86.9% None (many diffs) n/a
2 ccsbBroad304_05241 pLX_304 0% 89.7% 86.9% V5 (many diffs) n/a
3 TRCN0000475834 TGGGCCAAAGTAAAATCCATAGAT pLX_317 41% 89.7% 86.9% V5 (many diffs) n/a
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