Transcript: Human NM_001253772.2

Homo sapiens synaptotagmin 6 (SYT6), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
SYT6 (148281)
Length:
4472
CDS:
98..1630

Additional Resources:

NCBI RefSeq record:
NM_001253772.2
NBCI Gene record:
SYT6 (148281)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001253772.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380602 GGAAACCCTCGGTTGTGATTT pLKO_005 1613 CDS 100% 13.200 18.480 N SYT6 n/a
2 TRCN0000379736 GGCTCACCCTCACAGTGATTA pLKO_005 1227 CDS 100% 13.200 18.480 N SYT6 n/a
3 TRCN0000236303 TAGTCTGGTAGTCCTACATTT pLKO_005 3484 3UTR 100% 13.200 18.480 N SYT6 n/a
4 TRCN0000236306 CACTCCTTGGTGGAGGTAAAG pLKO_005 1577 CDS 100% 10.800 15.120 N SYT6 n/a
5 TRCN0000155746 CGATGGACATCACAGGCTATT pLKO.1 1266 CDS 100% 10.800 15.120 N SYT6 n/a
6 TRCN0000236302 TTCAGCCTACGCTACGATTAC pLKO_005 800 CDS 100% 10.800 15.120 N SYT6 n/a
7 TRCN0000154956 GCATCTCAGTGTCTTCGACTT pLKO.1 1033 CDS 100% 4.050 5.670 N SYT6 n/a
8 TRCN0000154675 GATCAACTTCAGCCTACGCTA pLKO.1 793 CDS 100% 2.640 3.696 N SYT6 n/a
9 TRCN0000236304 ATCTGGAAGGATATCCAATAT pLKO_005 1139 CDS 100% 13.200 10.560 N SYT6 n/a
10 TRCN0000380627 ATGAGATGTGCAGCCAAATAA pLKO_005 1750 3UTR 100% 15.000 10.500 N SYT6 n/a
11 TRCN0000380878 GCACTTGTTCAACCGTCTAAA pLKO_005 1800 3UTR 100% 13.200 9.240 N SYT6 n/a
12 TRCN0000236305 ATCTCAGTGTCTTCGACTTTG pLKO_005 1035 CDS 100% 10.800 7.560 N SYT6 n/a
13 TRCN0000380753 ATGTCTCCAGTGTAGACTATG pLKO_005 648 CDS 100% 10.800 7.560 N SYT6 n/a
14 TRCN0000381748 GATGGACATCACAGGCTATTC pLKO_005 1267 CDS 100% 10.800 7.560 N SYT6 n/a
15 TRCN0000175010 GAGGTAAAGAAATCCTTCAAA pLKO.1 1589 CDS 100% 5.625 3.938 N Syt6 n/a
16 TRCN0000154596 GCATGTCTCCAGTGTAGACTA pLKO.1 646 CDS 100% 4.950 3.465 N SYT6 n/a
17 TRCN0000156189 CACAAGTGAAAGCGTGGACTT pLKO.1 1162 CDS 100% 4.050 2.835 N SYT6 n/a
18 TRCN0000154940 GCTCATCTCAGTCATGGACTA pLKO.1 1435 CDS 100% 4.050 2.835 N SYT6 n/a
19 TRCN0000154644 CCGTCTAAACAGTGTTGTGCA pLKO.1 1812 3UTR 100% 2.640 1.848 N SYT6 n/a
20 TRCN0000380698 CTCTCAATCCTGTCTACAATG pLKO_005 1365 CDS 100% 10.800 6.480 N SYT6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001253772.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05010 pDONR223 100% 83.3% 83.3% None 1_255del n/a
2 ccsbBroad304_05010 pLX_304 0% 83.3% 83.3% V5 1_255del n/a
3 TRCN0000471407 GGGTGCAAATAAAATGAGCCCCAC pLX_317 29.2% 83.3% 83.3% V5 1_255del n/a
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