Construct: ORF ccsbBroad304_05010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008521.1_s304c1, BRDN0000410765
- Derived from:
- ccsbBroadEn_05010
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT6 (148281)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05010
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148281 | SYT6 | synaptotagmin 6 | NM_001270805.1 | 100% | 100% | |
| 2 | human | 148281 | SYT6 | synaptotagmin 6 | NM_001366223.1 | 100% | 100% | |
| 3 | human | 148281 | SYT6 | synaptotagmin 6 | NM_205848.3 | 100% | 100% | |
| 4 | human | 148281 | SYT6 | synaptotagmin 6 | NM_001366226.1 | 95.4% | 95% | (many diffs) |
| 5 | human | 148281 | SYT6 | synaptotagmin 6 | XM_024453337.1 | 95.4% | 95% | (many diffs) |
| 6 | human | 148281 | SYT6 | synaptotagmin 6 | NM_001253772.2 | 83.3% | 83.3% | 1_255del |
| 7 | human | 148281 | SYT6 | synaptotagmin 6 | XM_024453336.1 | 82.1% | 81.7% | (many diffs) |
| 8 | human | 148281 | SYT6 | synaptotagmin 6 | NM_001366225.1 | 81.9% | 81.9% | 1_255del;1491_1492ins21 |
| 9 | human | 148281 | SYT6 | synaptotagmin 6 | NM_001366224.1 | 80.1% | 79.7% | (many diffs) |
| 10 | human | 148281 | SYT6 | synaptotagmin 6 | XR_001736985.1 | 29.7% | 1_166del;1442_4283del | |
| 11 | human | 148281 | SYT6 | synaptotagmin 6 | XR_002959489.1 | 21.5% | 1_1797del;3073_5914del | |
| 12 | human | 148281 | SYT6 | synaptotagmin 6 | XR_946547.3 | 21.2% | 1_1797del;3033_3034ins21;3052_5893del | |
| 13 | mouse | 54524 | Syt6 | synaptotagmin VI | NM_001276677.1 | 90.2% | 96.7% | (many diffs) |
| 14 | mouse | 54524 | Syt6 | synaptotagmin VI | NM_001276679.1 | 90.2% | 96.7% | (many diffs) |
| 15 | mouse | 54524 | Syt6 | synaptotagmin VI | NM_001276681.1 | 90.2% | 96.7% | (many diffs) |
| 16 | mouse | 54524 | Syt6 | synaptotagmin VI | NM_001276676.1 | 86.4% | 92.1% | (many diffs) |
| 17 | mouse | 54524 | Syt6 | synaptotagmin VI | XM_006501706.2 | 86.4% | 92.1% | (many diffs) |
| 18 | mouse | 54524 | Syt6 | synaptotagmin VI | XM_006501707.2 | 86.4% | 92.1% | (many diffs) |
| 19 | mouse | 54524 | Syt6 | synaptotagmin VI | XM_006501708.2 | 86.4% | 92.1% | (many diffs) |
| 20 | mouse | 54524 | Syt6 | synaptotagmin VI | NM_018800.4 | 75.2% | 80.6% | (many diffs) |
| 21 | mouse | 54524 | Syt6 | synaptotagmin VI | XM_006501705.2 | 72.7% | 76.2% | (many diffs) |
| 22 | mouse | 54524 | Syt6 | synaptotagmin VI | XM_006501704.3 | 72.6% | 77.3% | (many diffs) |
| 23 | mouse | 54524 | Syt6 | synaptotagmin VI | NM_001276680.1 | 71.9% | 71.4% | (many diffs) |
| 24 | mouse | 54524 | Syt6 | synaptotagmin VI | XR_375547.3 | 26.4% | (many diffs) | |
| 25 | mouse | 54524 | Syt6 | synaptotagmin VI | XR_375545.2 | 25.9% | (many diffs) | |
| 26 | mouse | 54524 | Syt6 | synaptotagmin VI | XR_375546.3 | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctggaggaac aaggaggcct ccagtccctc ttctgctaat ccccccttgg 121 aagccctcca gagccccagc ttcagaggca acatggcgga caagctgaag gaccccagca 181 ccctgggctt cctggaggcg gccgtgaaga tcagccacac gtccccagat atcccagctg 241 aggtgcagat gtcggtcaag gagcacatca tgcgtcacac ccggctgcag cggcaaacta 301 cagagccagc gtcatccacc aggcacacgt ccttcaagcg ccacctgcca aggcagatgc 361 atgtctccag tgtagactat ggcaatgagc ttccaccagc agcagagcag cccaccagca 421 ttggccgcat caagcctgag ctctacaagc agaagtcggt ggatggggag gatgccaagt 481 ctgaggccac caagagctgc gggaagatca acttcagcct acgctacgat tacgagaccg 541 agaccctgat tgtgcgtatc ctgaaggctt ttgacctccc tgccaaggac ttttgtggaa 601 gctctgaccc ttatgtcaag atctacctcc tgcctgaccg caaatgcaag ctgcagaccc 661 gggtgcaccg caagaccctg aaccccacct ttgatgagaa cttccacttc cctgtgccct 721 atgaggagct ggctgaccgc aagctgcatc tcagtgtctt cgactttgac cgcttctccc 781 gccatgacat gattggcgag gtcatcctgg acaacctctt tgaggcctct gacctgtctc 841 gggaaacctc catctggaag gatatccaat atgccacaag tgaaagcgtg gacttgggag 901 agatcatgtt ctccctttgc tacctgccca ctgcaggcag gctcaccctc acagtgatta 961 agtgtcggaa cctcaaggcg atggacatca caggctattc agatccctat gtgaaagtgt 1021 ccttgctctg tgatgggcgg aggctgaaga agaagaaaac aaccataaag aaaaacactc 1081 tcaatcctgt ctacaatgag gccatcatct ttgacattcc cccggaaaac atggatcaag 1141 tcagcctgct catctcagtc atggactatg atcgagtggg ccacaatgag atcataggag 1201 tctgtcgtgt ggggatcact gctgaaggcc tgggcaggga ccactggaac gagatgctgg 1261 catacccccg gaagcccatc gcacactggc actccttggt ggaggtaaag aaatccttca 1321 aagagggaaa ccctcggttg tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1381 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1441 ttaagtcgac aatcaacctc tggatta