Transcript: Mouse NM_001256383.1

Mus musculus regulating synaptic membrane exocytosis 2 (Rims2), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-11
Taxon:
Mus musculus (mouse)
Gene:
Rims2 (116838)
Length:
7338
CDS:
78..4730

Additional Resources:

NCBI RefSeq record:
NM_001256383.1
NBCI Gene record:
Rims2 (116838)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001256383.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106156 GCGGCCATAATTGTTCCTATT pLKO.1 388 CDS 100% 10.800 15.120 N Rims2 n/a
2 TRCN0000381790 TTACGCTCAAACAAGGTTATG pLKO_005 450 CDS 100% 10.800 15.120 N Rims2 n/a
3 TRCN0000106159 GCTGATACTGTAGGACATCTT pLKO.1 2103 CDS 100% 4.950 6.930 N Rims2 n/a
4 TRCN0000106157 GCCCAGATACTCTTAGATGAA pLKO.1 4566 CDS 100% 4.950 3.960 N Rims2 n/a
5 TRCN0000379581 CAAGATCTCCTTCGGATTATG pLKO_005 877 CDS 100% 13.200 9.240 N Rims2 n/a
6 TRCN0000106158 CGTGAACGAATGCTGGAAATT pLKO.1 2643 CDS 100% 13.200 9.240 N Rims2 n/a
7 TRCN0000382378 GCAGATGGATGCGGCCATAAT pLKO_005 378 CDS 100% 13.200 9.240 N Rims2 n/a
8 TRCN0000381485 TACGGAAACAGCACCACTTAG pLKO_005 1438 CDS 100% 10.800 7.560 N RIMS2 n/a
9 TRCN0000106155 CACAACAACCAGCGATACAAA pLKO.1 4749 3UTR 100% 5.625 3.938 N Rims2 n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 6649 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256383.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07470 pDONR223 100% 63.5% 65.5% None (many diffs) n/a
2 ccsbBroad304_07470 pLX_304 0% 63.5% 65.5% V5 (many diffs) n/a
3 TRCN0000473307 TTTTCTTAGGCCTTTTTCGTTTAA pLX_317 13.5% 63.5% 65.5% V5 (many diffs) n/a
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