Transcript: Human NM_001256423.2

Homo sapiens Rho GTPase activating protein 19 (ARHGAP19), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
ARHGAP19 (84986)
Length:
5424
CDS:
24..1481

Additional Resources:

NCBI RefSeq record:
NM_001256423.2
NBCI Gene record:
ARHGAP19 (84986)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000298660 TTTAGTGGGCAGGTGTTATTC pLKO_005 1524 3UTR 100% 13.200 7.920 N ARHGAP19 n/a
2 TRCN0000047241 CCCTCCTCCTAATCGTAATTT pLKO.1 701 CDS 100% 15.000 7.500 Y ARHGAP19 n/a
3 TRCN0000298661 ATCACAAGGCTCATCGATTTA pLKO_005 189 CDS 100% 13.200 6.600 Y ARHGAP19 n/a
4 TRCN0000293965 GTTTAGAGTACCGGGTAATAG pLKO_005 419 CDS 100% 13.200 6.600 Y ARHGAP19 n/a
5 TRCN0000298657 TGTGCGAGATTGCACTATTTG pLKO_005 945 CDS 100% 13.200 6.600 Y ARHGAP19 n/a
6 TRCN0000047240 CCTTCAGGAGAATATCACAAA pLKO.1 851 CDS 100% 4.950 2.475 Y ARHGAP19 n/a
7 TRCN0000047242 GAGCTGTTTCAACACGTTCAT pLKO.1 1119 CDS 100% 4.950 2.475 Y ARHGAP19 n/a
8 TRCN0000286580 GAGCTGTTTCAACACGTTCAT pLKO_005 1119 CDS 100% 4.950 2.475 Y ARHGAP19 n/a
9 TRCN0000047238 GCTGACACATAAACACTTCAA pLKO.1 569 CDS 100% 4.950 2.475 Y ARHGAP19 n/a
10 TRCN0000047239 CCATTGGTGAATTGAAGGGAA pLKO.1 1348 CDS 100% 2.640 1.320 Y ARHGAP19 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256423.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04462 pDONR223 100% 96.7% 96.3% None (many diffs) n/a
2 ccsbBroad304_04462 pLX_304 0% 96.7% 96.3% V5 (many diffs) n/a
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