Construct: ORF ccsbBroad304_04462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012634.1_s304c1, BRDN0000408584
- Derived from:
- ccsbBroadEn_04462
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP19 (84986)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04462
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84986 | ARHGAP19 | Rho GTPase activating prote... | NM_032900.6 | 99.9% | 100% | 822T>C |
| 2 | human | 84986 | ARHGAP19 | Rho GTPase activating prote... | NM_001256423.2 | 96.7% | 96.3% | (many diffs) |
| 3 | human | 84986 | ARHGAP19 | Rho GTPase activating prote... | NM_001204300.1 | 94% | 94.1% | 822T>C;840_841ins87 |
| 4 | human | 100533184 | ARHGAP19-SLIT1 | ARHGAP19-SLIT1 readthrough ... | NR_037909.1 | 77% | (many diffs) | |
| 5 | mouse | 71085 | Arhgap19 | Rho GTPase activating prote... | NM_027667.3 | 86.2% | 87.8% | (many diffs) |
| 6 | mouse | 71085 | Arhgap19 | Rho GTPase activating prote... | XM_011247341.1 | 83.4% | 85.6% | (many diffs) |
| 7 | mouse | 71085 | Arhgap19 | Rho GTPase activating prote... | NM_001163495.1 | 82% | 83.2% | (many diffs) |
| 8 | mouse | 71085 | Arhgap19 | Rho GTPase activating prote... | XM_011247342.1 | 79.4% | 81.4% | (many diffs) |
| 9 | mouse | 71085 | Arhgap19 | Rho GTPase activating prote... | XM_011247343.1 | 79.2% | 81% | (many diffs) |
| 10 | mouse | 71085 | Arhgap19 | Rho GTPase activating prote... | XM_011247344.2 | 77.7% | 76% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1551
- ORF length:
- 1482
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgactgag gcacagagtg aaggggaggt gccagcccgc gaatccggcc 121 ggagtgatgc catctgcagt tttgtgatct gcaatgattc ttcccttcga ggtcagccca 181 ttatctttaa tcctgacttt tttgtggaga aactccgaca tgagaaacct gagattttca 241 ctgagttggt ggtcagcaat atcacaaggc tcatcgattt acctggaact gagttggctc 301 agctgatggg ggaagtggac cttaagttgc ctggcggggc tggcccagca tcaggattct 361 tccggtctct catgtctctc aagcgaaagg aaaaaggagt gatatttggg tccccactga 421 cggaggaagg cattgcccag atataccaac tgattgagta tctacacaaa aacttgcgag 481 tagagggttt gtttagagta ccgggtaata gtgtccgaca gcagatttta agggatgctc 541 tcaataatgg aactgacatt gacttggaat caggggaatt tcactcaaat gatgttgcca 601 ctttgctgaa gatgtttcta ggagagttgc cggagcctct gctgacacat aaacacttca 661 atgcacacct caaaatcgct gatttgatgc agtttgatga taaaggaaac aagaccaata 721 taccagacaa ggaccggcaa attgaggctc tccagttgct cttcctcatt ctccctcctc 781 ctaatcgtaa tttgctgaag ttattgcttg atctcctata ccagacagca aagaaacaag 841 acaagaacaa gatgtcagcc tataaccttg cccttatgtt tgcaccccac gtcctgtggc 901 caaaaaatgt cactgcaaat gaccttcagg agaatatcac aaagttaaac agtgggatgg 961 cttttatgat taaacactcc cagaaacttt ttaaggctcc tgcttacatt cgggagtgtg 1021 cgagattgca ctatttggga tccagaactc aggcatcaaa ggatgacctt gacctcatag 1081 cttcatgtca tactaagtcc tttcagctgg caaagtctca gaaacggaac cgggtagatt 1141 cctgccctca ccaggaggag acccagcacc atacggaaga ggcactgaga gagctgtttc 1201 aacacgttca tgatatgcca gagtcagcaa agaagaaaca acttattaga cagtttaata 1261 agcaatcatt gacccagaca ccagggcgag aaccttctac ttcccaggta caaaagaggg 1321 ctcgttcgcg ctccttcagt gggcttatta agcggaaggt cctgggaaat cagatgatgt 1381 cagaaaagaa aaagaagaac cctactccag aatctgtggc cattggtgaa ttgaagggaa 1441 ccagcaaaga aaataggaac ttattatttt ctggctctcc agctgtcacg atgacaccaa 1501 caagattgaa gtggtctgaa gggaagaaag aggggaaaaa aggatttctc ttgccaactt 1561 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1621 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta