Transcript: Mouse NM_001267724.1

Mus musculus ankyrin repeat and SOCS box-containing 13 (Asb13), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Asb13 (142688)
Length:
1935
CDS:
102..371

Additional Resources:

NCBI RefSeq record:
NM_001267724.1
NBCI Gene record:
Asb13 (142688)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001267724.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000200992 CCTGGAACAAATGTAGTCATT pLKO.1 711 3UTR 100% 4.950 6.930 N Asb13 n/a
2 TRCN0000190059 GCTCATCGACTACCTTTCCTA pLKO.1 436 3UTR 100% 3.000 4.200 N Asb13 n/a
3 TRCN0000319722 GCTCATCGACTACCTTTCCTA pLKO_005 436 3UTR 100% 3.000 4.200 N Asb13 n/a
4 TRCN0000217877 GAATGCGTGAGGCTTCTTATT pLKO.1 153 CDS 100% 13.200 9.240 N Asb13 n/a
5 TRCN0000190033 GCAGCTTACAATGGCCCATTA pLKO.1 1164 3UTR 100% 10.800 7.560 N Asb13 n/a
6 TRCN0000350120 GCAGCTTACAATGGCCCATTA pLKO_005 1164 3UTR 100% 10.800 7.560 N Asb13 n/a
7 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1251 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001267724.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10251 pDONR223 100% 25.9% 28.7% None (many diffs) n/a
2 ccsbBroad304_10251 pLX_304 0% 25.9% 28.7% V5 (many diffs) n/a
3 TRCN0000481338 TGACCTCCATATCCGCAGTGCCGC pLX_317 92.5% 25.9% 28.7% V5 (many diffs) n/a
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