Construct: ORF TRCN0000481338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002825.1_s317c1
- Derived from:
- ccsbBroadEn_10251
- DNA Barcode:
- TGACCTCCATATCCGCAGTGCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB13 (79754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481338
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79754 | ASB13 | ankyrin repeat and SOCS box... | NM_024701.4 | 61.9% | 61.8% | 518_834delinsCT |
| 2 | human | 79754 | ASB13 | ankyrin repeat and SOCS box... | NR_037164.2 | 18.5% | 1_44del;564_2798del | |
| 3 | human | 79754 | ASB13 | ankyrin repeat and SOCS box... | NR_024581.2 | 13.4% | 1_44del;274_275ins151;411_2566delinsCT | |
| 4 | mouse | 142688 | Asb13 | ankyrin repeat and SOCS box... | XR_382012.3 | 55% | (many diffs) | |
| 5 | mouse | 142688 | Asb13 | ankyrin repeat and SOCS box... | NM_178283.4 | 54.3% | 60.7% | (many diffs) |
| 6 | mouse | 142688 | Asb13 | ankyrin repeat and SOCS box... | NM_001267724.1 | 25.9% | 28.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccccgggcg gcggacggct gcttccTGGG CGACGTGGGT TTCTGGGTGG 121 AGCGGACCCC TGTGCACGAG GCAGCCCAGC GGGGTGAGAG CCTGCAGCTG CAACAGCTGA 181 TCGAGAGCGG CGCCTGCGTG AACCAGGTCA CCGTGGACTC CATCACGCCC CTGCACGCAG 241 CCAGTCTGCA GGGCCAGGCG CGGTGTGTGC AGCTGCTGCT GGCGGCTGGG GCCCAGGTGG 301 ATGCTCGCAA CATCGACGGC AGCACCCCGC TCTGCGATGC CTGCGCCTCG GGCAGCATCG 361 AGTGTGTGAA GCTCTTGCTG TCCTACGGGG CCAAGGTCAA CCCTCCCCTG TACACAGCGT 421 CCCCCCTGCA CGAGGCCTGC ATGAGCGGGA GTTCCGAATG TGTGAGGCTT CTTATTGACG 481 TCGGGGCCAA TCTGGAAGCG CACGATTGCC ATTTTGGGAC CCCTCTGCAC GTTGCCTGTG 541 CCCGGGAGCA TCTGGACTGT GTCAAAGTGC TGCTCAATGC AGCTTGCCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA TGACCTCCAT ATCCGCAGTG CCGCACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt