Transcript: Human NM_001277818.1

Homo sapiens YTH N6-methyladenosine RNA binding protein 3 (YTHDF3), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
YTHDF3 (253943)
Length:
4931
CDS:
239..1843

Additional Resources:

NCBI RefSeq record:
NM_001277818.1
NBCI Gene record:
YTHDF3 (253943)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001277818.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365173 TAAGTCAAAGAAGACGTATTA pLKO_005 1905 3UTR 100% 13.200 18.480 N YTHDF3 n/a
2 TRCN0000353555 GATAAGTGGAAGGGCAAATTT pLKO_005 1568 CDS 100% 15.000 12.000 N Ythdf3 n/a
3 TRCN0000365164 GATAAGTGGAAGGGCAAATTT pLKO_005 1568 CDS 100% 15.000 12.000 N YTHDF3 n/a
4 TRCN0000370249 ATAACCAATTACGGCATATTC pLKO_005 1623 CDS 100% 13.200 9.240 N YTHDF3 n/a
5 TRCN0000370250 GAAGTCTGTTGTGGACTATAA pLKO_005 1522 CDS 100% 13.200 9.240 N YTHDF3 n/a
6 TRCN0000365133 TAATGGAGAACATCACTATAT pLKO_005 367 CDS 100% 13.200 9.240 N YTHDF3 n/a
7 TRCN0000172353 CAAGTGGATCTCAGGGACAAT pLKO.1 498 CDS 100% 4.950 3.465 N YTHDF3 n/a
8 TRCN0000167222 CCAGATGGTGTATTTAGTCAA pLKO.1 389 CDS 100% 4.950 3.465 N YTHDF3 n/a
9 TRCN0000167772 GCAAGGAAATAAAGTTTCAGT pLKO.1 121 5UTR 100% 3.000 2.100 N YTHDF3 n/a
10 TRCN0000370197 GCTACTCTGAGGATGACATAC pLKO_005 1353 CDS 100% 10.800 6.480 N YTHDF3 n/a
11 TRCN0000167348 CACAAAGTTCTGCTTATAGTA pLKO.1 522 CDS 100% 5.625 2.813 Y YTHDF3 n/a
12 TRCN0000191772 GCCTAATAAGTCAAAGAAGAT pLKO.1 1899 3UTR 100% 4.950 3.465 N Ythdf3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001277818.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05293 pDONR223 100% 91.2% 91.2% None 0_1ins153 n/a
2 ccsbBroad304_05293 pLX_304 0% 91.2% 91.2% V5 0_1ins153 n/a
3 TRCN0000473510 CAGACAGTATCCGGGCATGGCAGC pLX_317 29.8% 91.2% 91.2% V5 0_1ins153 n/a
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