Construct: ORF TRCN0000473510
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005720.1_s317c1
- Derived from:
- ccsbBroadEn_05293
- DNA Barcode:
- CAGACAGTATCCGGGCATGGCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YTHDF3 (253943)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473510
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_152758.6 | 100% | 100% | |
| 2 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_001277813.1 | 97.7% | 96.7% | (many diffs) |
| 3 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_001277814.1 | 97.7% | 96.7% | (many diffs) |
| 4 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | XM_011517509.3 | 97.7% | 96.7% | (many diffs) |
| 5 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_001277815.1 | 91.2% | 91.2% | 0_1ins153 |
| 6 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_001277816.1 | 91.2% | 91.2% | 0_1ins153 |
| 7 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_001277817.1 | 91.2% | 91.2% | 0_1ins153 |
| 8 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | NM_001277818.1 | 91.2% | 91.2% | 0_1ins153 |
| 9 | human | 253943 | YTHDF3 | YTH N6-methyladenosine RNA ... | XM_011517510.3 | 91.2% | 91.2% | 0_1ins153 |
| 10 | mouse | 229096 | Ythdf3 | YTH domain family 3 | NM_001145919.1 | 96.4% | 98.8% | (many diffs) |
| 11 | mouse | 229096 | Ythdf3 | YTH domain family 3 | NM_172677.3 | 94.6% | 96.9% | (many diffs) |
| 12 | mouse | 229096 | Ythdf3 | YTH domain family 3 | XM_006535442.3 | 92.6% | 93.8% | (many diffs) |
| 13 | mouse | 229096 | Ythdf3 | YTH domain family 3 | XM_006535443.3 | 87.8% | 90.2% | (many diffs) |
| 14 | mouse | 229096 | Ythdf3 | YTH domain family 3 | XM_006535444.2 | 87.8% | 90.2% | (many diffs) |
| 15 | mouse | 229096 | Ythdf3 | YTH domain family 3 | NR_027375.1 | 31.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1821
- ORF length:
- 1755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agccactagc gtggatcaga gacctaaagg gcaaggaaat aaagtttcag 121 tacaaaacgg ttcgattcat caaaaagatg ctgtaaatga tgatgatttt gagccatact 181 taagtagcca gacaaatcag agtaacagct atccaccaat gtcagatcca tacatgccta 241 gttactatgc tccatccatt ggatttccat attctcttgg ggaagcagcg tggtccacag 301 ctggagacca gcctatgcca tatctgacaa cctatggaca aatgagtaat ggagaacatc 361 actatatacc agatggtgta tttagtcaac ctggggcatt aggaaatacc cctccatttc 421 ttggtcaaca tggatttaac ttttttcctg gtaatgctga tttctctaca tgggggacaa 481 gtggatctca gggacaatca acacaaagtt ctgcttatag tagcagttat ggctatccac 541 ctagttctct tgggagagct attactgatg gacaggctgg atttggcaat gatactttga 601 gtaaggtgcc tggcattagc agtattgagc aaggcatgac tggactgaaa attggtggtg 661 acctgacagc tgcagtgaca aaaactgtag gtacagcttt gagcagcagt ggtatgacta 721 gcattgcaac caatagtgtg cccccagtta gcagtgcagc acctaaacca acctcctggg 781 ctgccattgc cagaaagcct gccaaacctc aaccgaaact taaacccaag ggcaatgtgg 841 gaattggggg ttctgctgta ccaccacctc ctataaaaca caacatgaat attggaactt 901 gggatgaaaa agggtcagtg gtaaaggctc caccaaccca accagttctg cctcctcaaa 961 ctataatcca gcagcctcag ccattaattc aaccaccacc attggtgcaa agccaactgc 1021 ctcaacagca gcctcaacca ccacaaccac agcagcaaca aggacctcag ccacaggccc 1081 agcctcacca agtgcagcct caacagcagc agctgcagaa tcgctgggta gctcctcgta 1141 acaggggagc aggcttcaac cagaacaatg gagcgggcag tgaaaacttt ggtttaggtg 1201 ttgtacctgt cagtgcttca ccttctagtg tagaagtgca tcccgtgctg gaaaagctaa 1261 aggccataaa caactataat cccaaagact ttgattggaa tcTGAAGAAT GGACGTGTGT 1321 TTATAATTAA AAGCTACTCT GAGGATGACA TACATCGTTC CATTAAATAC TCTATCTGGT 1381 GTAGTACTGA GCATGGTAAT AAGCGTTTGG ATGCAGCTTA CCGTTCCCTG AATGGGAAAG 1441 GCCCACTCTA TTTACTCTTC AGTGTGAATG GCAGTGGACA TTTTTGTGGA GTGGCTGAAA 1501 TGAAGTCTGT TGTGGACTAT AATGCGTATG CTGGTGTCTG GTCTCAGGAT AAGTGGAAGG 1561 GCAAATTTGA AGTTAAATGG ATCTTTGTCA AAGATGTTCC CAATAACCAA TTACGGCATA 1621 TTCGCTTAGA AAATAATGAC AACAAACCGG TTACCAATTC AAGGGACACT CAAGAGGTAC 1681 CCCTAGAAAA AGCTAAGCAA GTGCTTAAAA TAATTGCTAC TTTCAAGCAT ACCACCTCAA 1741 TCTTTGATGA CTTTGCACAT TATGAAAAGC GTCAAGAAGA GGAGGAAGCC ATGCGTAGGG 1801 AGAGAAATAG AAACAAACAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAGA CAGTATCCGG 1981 GCATGGCAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt