Transcript: Human NM_001291733.1

Homo sapiens post-GPI attachment to proteins phospholipase 3 (PGAP3), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-08-31
Taxon:
Homo sapiens (human)
Gene:
PGAP3 (93210)
Length:
2254
CDS:
57..503

Additional Resources:

NCBI RefSeq record:
NM_001291733.1
NBCI Gene record:
PGAP3 (93210)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001291733.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000167276 CGACTGTAAGTATGAGTGTAT pLKO.1 254 CDS 100% 4.950 3.960 N PGAP3 n/a
2 TRCN0000433431 TGGAACTGAGTGTGCTCTTAG pLKO_005 807 3UTR 100% 10.800 7.560 N PGAP3 n/a
3 TRCN0000168629 GAAGGAATCAGAGGACAAGTT pLKO.1 519 3UTR 100% 4.950 3.465 N PGAP3 n/a
4 TRCN0000167247 CAACTTCTTGAACTTGGACAT pLKO.1 645 3UTR 100% 4.050 2.835 N PGAP3 n/a
5 TRCN0000172278 CCAGCCAATCTACATGAGTCT pLKO.1 212 CDS 100% 2.640 1.848 N PGAP3 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001291733.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04598 pDONR223 100% 45.8% 45.3% None (many diffs) n/a
2 ccsbBroad304_04598 pLX_304 0% 45.8% 45.3% V5 (many diffs) n/a
3 TRCN0000465222 ACGCATCCCATACATCCGCCCGGC pLX_317 22.3% 45.8% 45.3% V5 (many diffs) n/a
Download CSV