Transcript: Mouse NM_001302343.1

Mus musculus nuclear factor, erythroid derived 2 (Nfe2), transcript variant 6, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Nfe2 (18022)
Length:
1527
CDS:
167..1288

Additional Resources:

NCBI RefSeq record:
NM_001302343.1
NBCI Gene record:
Nfe2 (18022)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001302343.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425519 CACTGCCACCTCCAACATATT pLKO_005 342 CDS 100% 13.200 10.560 N Nfe2 n/a
2 TRCN0000081984 GCAGAGCTGTACCATGATATT pLKO.1 1145 CDS 100% 13.200 10.560 N Nfe2 n/a
3 TRCN0000433121 ACTGAGCTGCAGGGTCTAAAT pLKO_005 269 CDS 100% 13.200 9.240 N Nfe2 n/a
4 TRCN0000081983 CCATATCACCTGGGAGTATTT pLKO.1 1400 3UTR 100% 13.200 9.240 N Nfe2 n/a
5 TRCN0000081985 CCAGAATCTGACTCAGGATTA pLKO.1 590 CDS 100% 10.800 7.560 N Nfe2 n/a
6 TRCN0000081987 CTTACTCACTTATGCCCAATT pLKO.1 708 CDS 100% 10.800 7.560 N Nfe2 n/a
7 TRCN0000424174 TTTGGCCCATCCCAACTATAC pLKO_005 730 CDS 100% 10.800 7.560 N Nfe2 n/a
8 TRCN0000081986 CCTTACTCACTTATGCCCAAT pLKO.1 707 CDS 100% 4.050 2.835 N Nfe2 n/a
9 TRCN0000430469 TCTGATGAAGGCACTTCTTAA pLKO_005 1336 3UTR 100% 13.200 7.920 N Nfe2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001302343.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01089 pDONR223 100% 85.3% 88.7% None (many diffs) n/a
2 ccsbBroad304_01089 pLX_304 0% 85.3% 88.7% V5 (many diffs) n/a
3 TRCN0000465520 CATACCCCTGGGACACATCATTGA pLX_317 22.9% 85.3% 88.7% V5 (many diffs) n/a
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