Construct: ORF TRCN0000465520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002896.1_s317c1
- Derived from:
- ccsbBroadEn_01089
- DNA Barcode:
- CATACCCCTGGGACACATCATTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFE2 (4778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4778 | NFE2 | nuclear factor, erythroid 2 | NM_001136023.3 | 100% | 100% | |
| 2 | human | 4778 | NFE2 | nuclear factor, erythroid 2 | NM_001261461.1 | 100% | 100% | |
| 3 | human | 4778 | NFE2 | nuclear factor, erythroid 2 | NM_006163.2 | 100% | 100% | |
| 4 | human | 4778 | NFE2 | nuclear factor, erythroid 2 | XM_005268906.4 | 100% | 100% | |
| 5 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | NM_001302338.1 | 85.3% | 88.7% | (many diffs) |
| 6 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | NM_001302339.1 | 85.3% | 88.7% | (many diffs) |
| 7 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | NM_001302340.1 | 85.3% | 88.7% | (many diffs) |
| 8 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | NM_001302341.1 | 85.3% | 88.7% | (many diffs) |
| 9 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | NM_001302343.1 | 85.3% | 88.7% | (many diffs) |
| 10 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | NM_008685.3 | 85.3% | 88.7% | (many diffs) |
| 11 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | XM_006520573.2 | 85.3% | 88.7% | (many diffs) |
| 12 | mouse | 18022 | Nfe2 | nuclear factor, erythroid d... | XM_006520569.2 | 80.4% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cccgtgtcct ccccagcaga gcaggaacag ggtgatacag ctgtccactt 121 cagagctagg agagatggaa ctgacttggc aggagatcat gtccatcacc gagctgcagg 181 gtctgaatgc tccaagtgag ccatcatttg agccccaagc cccagctcca taccttggac 241 ctccaccacc cacaacttac tgcccctgct caatccaccc agattctggc ttcccacttc 301 ctccaccacc ttatgagctc ccagcatcca catcccatgt cccagatccc ccatactcct 361 atggcaacat ggccatacca gtctccaagc cactgagcct ctcaggcctg ctcagtgagc 421 cgctccaaga ccccttagcc ctcctggaca ttgggctgcc agcagggcca cctaagcccc 481 aagaagaccc agaatccgac tcaggattat ccctcaacta tagcgatgct gaatctcttg 541 agctggaggg gacagaggct ggtcggcggc gcagcgaata tgtagagatg tacccagtgg 601 agtaccccta ctcactcatg cccaactcct tggcccactc caactatacc ttgccagctg 661 ctgagacccc cttggcctta gagccctcct caggccctgt gcgggctaag cccactgcac 721 ggggggaggc agggagtcgg gatgaacgtc gggccttggc catgaagatt ccttttccta 781 cggacaagat tgtcaacttg ccggtagatg actttaatga gctattggca aggtacccgc 841 tgacagagag ccagctagcg ctagtccggg acatccgacg acggggcaaa aacaaggtgg 901 cagcccagaa ctgccgcaag aggaagctgg aaacCATTGT GCAGCTGGAG CGGGAGCTGG 961 AGCGGCTGAC CAATGAACGG GAGCGGCTTC TCAGGGCCCG CGGGGAGGCA GACCGGACCC 1021 TGGAGGTCAT GCGCCAACAG CTGACAGAGC TGTACCGTGA CATTTTCCAG CACCTTCGGG 1081 ATGAATCAGG CAACAGCTAC TCTCCTGAAG AGTACGCGCT GCAACAGGCT GCCGATGGGA 1141 CCATCTTCCT TGTGCCCCGG GGGACCAAGA TGGAGGCCAC AGACTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA CATACCCCTG GGACACATCA TTGAACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt