Construct: ORF TRCN0000465520

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002896.1_s317c1
Derived from:
ccsbBroadEn_01089
DNA Barcode:
CATACCCCTGGGACACATCATTGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NFE2 (4778)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465520
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4778 NFE2 nuclear factor, erythroid 2 NM_001136023.3 100% 100%
2 human 4778 NFE2 nuclear factor, erythroid 2 NM_001261461.1 100% 100%
3 human 4778 NFE2 nuclear factor, erythroid 2 NM_006163.2 100% 100%
4 human 4778 NFE2 nuclear factor, erythroid 2 XM_005268906.4 100% 100%
5 mouse 18022 Nfe2 nuclear factor, erythroid d... NM_001302338.1 85.3% 88.7% (many diffs)
6 mouse 18022 Nfe2 nuclear factor, erythroid d... NM_001302339.1 85.3% 88.7% (many diffs)
7 mouse 18022 Nfe2 nuclear factor, erythroid d... NM_001302340.1 85.3% 88.7% (many diffs)
8 mouse 18022 Nfe2 nuclear factor, erythroid d... NM_001302341.1 85.3% 88.7% (many diffs)
9 mouse 18022 Nfe2 nuclear factor, erythroid d... NM_001302343.1 85.3% 88.7% (many diffs)
10 mouse 18022 Nfe2 nuclear factor, erythroid d... NM_008685.3 85.3% 88.7% (many diffs)
11 mouse 18022 Nfe2 nuclear factor, erythroid d... XM_006520573.2 85.3% 88.7% (many diffs)
12 mouse 18022 Nfe2 nuclear factor, erythroid d... XM_006520569.2 80.4% 83.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1185
ORF length:
1119
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc cccgtgtcct ccccagcaga gcaggaacag ggtgatacag ctgtccactt
121cagagctagg agagatggaa ctgacttggc aggagatcat gtccatcacc gagctgcagg
181gtctgaatgc tccaagtgag ccatcatttg agccccaagc cccagctcca taccttggac
241ctccaccacc cacaacttac tgcccctgct caatccaccc agattctggc ttcccacttc
301ctccaccacc ttatgagctc ccagcatcca catcccatgt cccagatccc ccatactcct
361atggcaacat ggccatacca gtctccaagc cactgagcct ctcaggcctg ctcagtgagc
421cgctccaaga ccccttagcc ctcctggaca ttgggctgcc agcagggcca cctaagcccc
481aagaagaccc agaatccgac tcaggattat ccctcaacta tagcgatgct gaatctcttg
541agctggaggg gacagaggct ggtcggcggc gcagcgaata tgtagagatg tacccagtgg
601agtaccccta ctcactcatg cccaactcct tggcccactc caactatacc ttgccagctg
661ctgagacccc cttggcctta gagccctcct caggccctgt gcgggctaag cccactgcac
721ggggggaggc agggagtcgg gatgaacgtc gggccttggc catgaagatt ccttttccta
781cggacaagat tgtcaacttg ccggtagatg actttaatga gctattggca aggtacccgc
841tgacagagag ccagctagcg ctagtccggg acatccgacg acggggcaaa aacaaggtgg
901cagcccagaa ctgccgcaag aggaagctgg aaacCATTGT GCAGCTGGAG CGGGAGCTGG
961AGCGGCTGAC CAATGAACGG GAGCGGCTTC TCAGGGCCCG CGGGGAGGCA GACCGGACCC
1021TGGAGGTCAT GCGCCAACAG CTGACAGAGC TGTACCGTGA CATTTTCCAG CACCTTCGGG
1081ATGAATCAGG CAACAGCTAC TCTCCTGAAG AGTACGCGCT GCAACAGGCT GCCGATGGGA
1141CCATCTTCCT TGTGCCCCGG GGGACCAAGA TGGAGGCCAC AGACTGCCCA ACTTTCTTGT
1201ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1261AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1321GAAAGGACGA CATACCCCTG GGACACATCA TTGAACGCGT TAAGTCgaca atcaacctct
1381ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)