Transcript: Mouse NM_001302951.1

Mus musculus transducin-like enhancer of split 4 (Tle4), transcript variant 4, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Tle4 (21888)
Length:
4067
CDS:
87..2405

Additional Resources:

NCBI RefSeq record:
NM_001302951.1
NBCI Gene record:
Tle4 (21888)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001302951.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000098289 CCTCCCAATTAAGGATGAGAA pLKO.1 626 CDS 100% 4.950 6.930 N Tle4 n/a
2 TRCN0000098286 CGGCATTATGTCATGTATTAT pLKO.1 273 CDS 100% 15.000 12.000 N Tle4 n/a
3 TRCN0000160491 CCAGAAACATTGTGTTATCTT pLKO.1 3515 3UTR 100% 5.625 4.500 N TLE4 n/a
4 TRCN0000098285 CGCTGTGCAAAGCAAGTCTTT pLKO.1 3337 3UTR 100% 4.950 3.465 N Tle4 n/a
5 TRCN0000098287 CCTCCAAATCTAACAGGCATT pLKO.1 1389 CDS 100% 4.050 2.835 N Tle4 n/a
6 TRCN0000098288 CCAGACAAATACCAGTTGCAT pLKO.1 2157 CDS 100% 3.000 2.100 N Tle4 n/a
7 TRCN0000165843 GCAGAACTGAACGCCATCATT pLKO.1 447 CDS 100% 5.625 3.375 N TLE4 n/a
8 TRCN0000275237 GCAGAACTGAACGCCATCATT pLKO_005 447 CDS 100% 5.625 3.375 N TLE4 n/a
9 TRCN0000166665 CCAAAGAGACAGAGACTCCAT pLKO.1 668 CDS 100% 2.640 1.584 N TLE4 n/a
10 TRCN0000275190 CCAAAGAGACAGAGACTCCAT pLKO_005 668 CDS 100% 2.640 1.584 N TLE4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001302951.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11192 pDONR223 100% 83.1% 88.8% None (many diffs) n/a
2 ccsbBroad304_11192 pLX_304 0% 83.1% 88.8% V5 (many diffs) n/a
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