Transcript: Human NM_001308195.2

Homo sapiens SUMO interacting motifs containing 1 (SIMC1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
SIMC1 (375484)
Length:
3305
CDS:
86..2761

Additional Resources:

NCBI RefSeq record:
NM_001308195.2
NBCI Gene record:
SIMC1 (375484)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308195.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000128452 GCCCTCTACTTTCTGAATAAT pLKO.1 2369 CDS 100% 15.000 21.000 N SIMC1 n/a
2 TRCN0000417330 TCGACCGAAAGGACTTAATAA pLKO_005 2520 CDS 100% 15.000 21.000 N SIMC1 n/a
3 TRCN0000415364 GTGGCTGGTCAAAGCAGTAAC pLKO_005 1987 CDS 100% 10.800 15.120 N SIMC1 n/a
4 TRCN0000148471 CCACAATGTCAGGGATGTTAT pLKO.1 1963 CDS 100% 13.200 9.240 N SIMC1 n/a
5 TRCN0000417664 GCTCAAGTGTCAGTCAGATAA pLKO_005 2401 CDS 100% 13.200 9.240 N SIMC1 n/a
6 TRCN0000422185 AGACCCTAGAAGATGACTTTC pLKO_005 1866 CDS 100% 10.800 7.560 N SIMC1 n/a
7 TRCN0000425339 CAGGAACAGGAATCTTGAAAG pLKO_005 2052 CDS 100% 10.800 7.560 N SIMC1 n/a
8 TRCN0000422025 TAGACGTAGAGAAGCAGATTG pLKO_005 2589 CDS 100% 10.800 7.560 N SIMC1 n/a
9 TRCN0000435913 TGGCAGACTTGGGACGAATTG pLKO_005 2429 CDS 100% 10.800 7.560 N SIMC1 n/a
10 TRCN0000432219 CCGAGATGATGTTTGGGTTTG pLKO_005 2208 CDS 100% 6.000 4.200 N SIMC1 n/a
11 TRCN0000146627 CCTGTTCTCAAATATGGCTTA pLKO.1 2939 3UTR 100% 4.050 2.835 N SIMC1 n/a
12 TRCN0000149214 GCACGGAGAATCATTAACAGT pLKO.1 446 CDS 100% 3.000 1.800 N SIMC1 n/a
13 TRCN0000149462 GCCTGTTCTCAAATATGGCTT pLKO.1 2938 3UTR 100% 2.640 1.584 N SIMC1 n/a
14 TRCN0000128288 GCCACTCTTGACTTAACTTTA pLKO.1 317 CDS 100% 13.200 6.600 Y SIMC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308195.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14485 pDONR223 100% 36.7% 36.2% None (many diffs) n/a
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