Construct: ORF ccsbBroadEn_14485

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004530.1_s300c1, BRDN0000393593
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SIMC1 (375484)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 375484 SIMC1 SUMO interacting motifs con... NM_001308200.1 99.8% 99.6% 13T>A
2 human 375484 SIMC1 SUMO interacting motifs con... NM_198567.5 71.6% 70.6% (many diffs)
3 human 375484 SIMC1 SUMO interacting motifs con... NR_131772.2 52.5% (many diffs)
4 human 375484 SIMC1 SUMO interacting motifs con... XM_017009455.1 41.8% 41.2% (many diffs)
5 human 375484 SIMC1 SUMO interacting motifs con... XM_017009456.1 41.8% 41.2% (many diffs)
6 human 375484 SIMC1 SUMO interacting motifs con... XM_011534556.2 37.7% 37.2% (many diffs)
7 human 375484 SIMC1 SUMO interacting motifs con... NM_001308196.1 37.5% 37% (many diffs)
8 human 375484 SIMC1 SUMO interacting motifs con... XM_017009454.1 37.5% 37% (many diffs)
9 human 375484 SIMC1 SUMO interacting motifs con... NM_001308195.2 36.7% 36.2% (many diffs)
10 human 375484 SIMC1 SUMO interacting motifs con... XM_011534553.2 36.5% 36% (many diffs)
11 human 375484 SIMC1 SUMO interacting motifs con... XM_011534554.2 36.5% 36% (many diffs)
12 mouse 319719 Simc1 SUMO-interacting motifs con... XM_006517277.1 80% 83.7% (many diffs)
13 mouse 319719 Simc1 SUMO-interacting motifs con... XM_017315526.1 80% 83.7% (many diffs)
14 mouse 319719 Simc1 SUMO-interacting motifs con... XM_011244530.1 22.1% 22.4% (many diffs)
15 mouse 319719 Simc1 SUMO-interacting motifs con... XM_011244531.1 22.1% 22.4% (many diffs)
16 mouse 319719 Simc1 SUMO-interacting motifs con... XM_011244529.1 20.9% 21.2% (many diffs)
17 mouse 319719 Simc1 SUMO-interacting motifs con... XM_011244528.1 20.4% 20.6% (many diffs)
18 mouse 319719 Simc1 SUMO-interacting motifs con... NM_176987.4 20.3% 21.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1065
ORF length:
999
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC TAGATCCATT GAACAAGTAA TAATACTTAA AAAATGGTTT CTGAAACCTT
121ATAAGGGACA AACTCTGCCT GGGCGAGTCC TTTTCCTGCG TTATGTCGTT CAGACCCTAG
181AAGATGACTT TCAGCAGACC CTGAGGAGGC AACGGCAGCA CCTGCAGCAA TCCATTGCAA
241ACATGGTGCT TTCCTGTGAC AAGCAGCCCC ACAATGTCAG GGATGTTATC AAGTGGCTGG
301TCAAAGCAGT AACTGAAGAT GGATTGACTC AGCCCCCAAA TGGAAATCAA ACGTCTTCAG
361GAACAGGAAT CTTGAAAGCC AGCAGTAGCC ACCCTTCTTC CCAGCCCAAC CTGACAAAGA
421ACACCAATCA GCTGATTGTG TGCCAGCTTC AGAGGATGCT CTCCATAGCC GTAGAGGTGG
481ACAGGACCCC CACCTGCAGC TCCAATAAAA TTGCCGAGAT GATGTTTGGG TTTGTGCTGG
541ACATTCCTGA GAGGAGCCAG AGAGAAATGT TCTTTACTAC CATGGAAAGC CACCTTCTGC
601GCTGCAAAGT GTTAGAAATC ATATTCCTCC ACAGCTGTGA GACACCCACC CGCCTGCCTC
661TGTCTCTGGC CCAGGCCCTC TACTTTCTGA ATAATTCTAC GTCACTGCTC AAGTGTCAGT
721CAGATAAAAG CCAGTGGCAG ACTTGGGACG AATTGGTTGA GCATCTGCAG TTTCTGCTGT
781CCAGTTATCA ACATGTTTTA AGAGAACACT TAAGGAGTTC CGTGATCGAC CGAAAGGACT
841TAATAATCAA AAGGATTAAG CCCAAACCCC AGCAAGGAGA TGACATCACA GTGGTAGACG
901TAGAGAAGCA GATTGAGGCC TTCCGCAGCC GCCTGATCCA GATGCTGGGG GAGCCTCTTG
961TCCCCCAACT CCAAGACAAA GTGCACTTGT TGAAGCTCCT GCTCTTCTAT GCTGCGGACT
1021TGAACCCTGA TGCAGAGCCC TTTCAAAAGG GCTGGAGCGG CTCCTGCCCA ACTTTCTAGT
1081ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)