Transcript: Human NM_001321812.1

Homo sapiens RAD51 paralog B (RAD51B), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
RAD51B (5890)
Length:
2938
CDS:
4..1059

Additional Resources:

NCBI RefSeq record:
NM_001321812.1
NBCI Gene record:
RAD51B (5890)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321812.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000154303 CCCAAAGATGCAAACGGCTTA pLKO.1 186 CDS 100% 4.050 5.670 N RAD51B n/a
2 TRCN0000152685 GCAAACGGCTTATGGGATAAA pLKO.1 195 CDS 100% 13.200 9.240 N RAD51B n/a
3 TRCN0000368588 GGCATCCTCCTTGAAGTATTT pLKO_005 717 CDS 100% 13.200 9.240 N RAD51B n/a
4 TRCN0000378334 TTGACACAGAGTCTGCATTTA pLKO_005 425 CDS 100% 13.200 9.240 N RAD51B n/a
5 TRCN0000364158 CCAAAGATGCAAACGGCTTAT pLKO_005 187 CDS 100% 10.800 7.560 N RAD51B n/a
6 TRCN0000151962 CCTGTGATGAAGTTCTACAAA pLKO.1 554 CDS 100% 5.625 3.938 N RAD51B n/a
7 TRCN0000152065 CAACTTCAAGGCAATCTCAAA pLKO.1 670 CDS 100% 4.950 3.465 N RAD51B n/a
8 TRCN0000153780 CCCAGTTATCTTGACGAATCA pLKO.1 756 CDS 100% 4.950 3.465 N RAD51B n/a
9 TRCN0000156231 CTGCTGATTTCTCACCAGCAT pLKO.1 227 CDS 100% 0.264 0.185 N RAD51B n/a
10 TRCN0000071263 CCAGTTATCTTGACGAATCAA pLKO.1 757 CDS 100% 5.625 4.500 N Rad51b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321812.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01369 pDONR223 100% 90.4% 89.8% None (many diffs) n/a
2 ccsbBroad304_01369 pLX_304 0% 90.4% 89.8% V5 (many diffs) n/a
3 TRCN0000470293 GTCAGAGCTCCATAACTAGATTTA pLX_317 32% 90.4% 89.8% V5 (many diffs) n/a
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