Transcript: Human NM_001322062.1

Homo sapiens zinc finger and SCAN domain containing 5A (ZSCAN5A), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-07-01
Taxon:
Homo sapiens (human)
Gene:
ZSCAN5A (79149)
Length:
2083
CDS:
147..1793

Additional Resources:

NCBI RefSeq record:
NM_001322062.1
NBCI Gene record:
ZSCAN5A (79149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322062.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000285248 GATTGCATAATCCGGTCTATG pLKO_005 1792 CDS 100% 10.800 15.120 N ZSCAN5A n/a
2 TRCN0000016769 CGAGAAGAGGTTTACGTGTAA pLKO.1 1385 CDS 100% 4.950 6.930 N ZSCAN5A n/a
3 TRCN0000274806 CGAGAAGAGGTTTACGTGTAA pLKO_005 1385 CDS 100% 4.950 6.930 N ZSCAN5A n/a
4 TRCN0000016770 GCTGAAATTGTTAAGACGCCA pLKO.1 1739 CDS 100% 0.660 0.924 N ZSCAN5A n/a
5 TRCN0000016771 CCTTCCACGGAAAGGAATATA pLKO.1 700 CDS 100% 15.000 10.500 N ZSCAN5A n/a
6 TRCN0000016768 GCAGCTTATTAGCCTCCAATT pLKO.1 1484 CDS 100% 10.800 7.560 N ZSCAN5A n/a
7 TRCN0000274874 GCAGCTTATTAGCCTCCAATT pLKO_005 1484 CDS 100% 10.800 7.560 N ZSCAN5A n/a
8 TRCN0000274873 TTGGAGCTGCCACGGTCTATG pLKO_005 363 CDS 100% 3.600 2.520 N ZSCAN5A n/a
9 TRCN0000285246 CAGAGGGAGTGCCCTAGATAT pLKO_005 1896 3UTR 100% 13.200 7.920 N ZSCAN5A n/a
10 TRCN0000225755 CCGGAGAGAAACCCTACAAAT pLKO_005 1690 CDS 100% 13.200 6.600 Y Zfp808 n/a
11 TRCN0000230663 CCCAATCTGGTGAGAGCAAAG pLKO_005 1041 CDS 100% 6.000 3.000 Y ZSCAN5B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322062.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04057 pDONR223 100% 90.1% 89.9% None 1_159del;898_899insCAG n/a
2 ccsbBroad304_04057 pLX_304 0% 90.1% 89.9% V5 1_159del;898_899insCAG n/a
3 TRCN0000469478 CGCTGCCAATCCTCCGATCTCTAG pLX_317 26% 90.1% 89.9% V5 1_159del;898_899insCAG n/a
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