Construct: ORF TRCN0000469478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018253.1_s317c1
- Derived from:
- ccsbBroadEn_04057
- DNA Barcode:
- CGCTGCCAATCCTCCGATCTCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN5A (79149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469478
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322064.2 | 100% | 100% | |
| 2 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322065.2 | 100% | 100% | |
| 3 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322066.1 | 100% | 100% | |
| 4 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322067.1 | 100% | 100% | |
| 5 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322068.1 | 100% | 100% | |
| 6 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322070.1 | 100% | 100% | |
| 7 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322072.1 | 100% | 100% | |
| 8 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_024303.2 | 100% | 100% | |
| 9 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322073.2 | 99.7% | 99.5% | 739_740insCAG |
| 10 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322074.2 | 99.7% | 99.5% | 739_740insCAG |
| 11 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322075.1 | 99.7% | 99.5% | 739_740insCAG |
| 12 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322076.1 | 99.7% | 99.5% | 739_740insCAG |
| 13 | human | 649137 | ZSCAN5C | zinc finger and SCAN domain... | NM_001358413.1 | 91% | 83.6% | (many diffs) |
| 14 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322061.2 | 90.1% | 89.9% | 1_159del;898_899insCAG |
| 15 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322062.1 | 90.1% | 89.9% | 1_159del;898_899insCAG |
| 16 | human | 342933 | ZSCAN5B | zinc finger and SCAN domain... | NM_001080456.2 | 89.2% | 79% | (many diffs) |
| 17 | human | 342933 | ZSCAN5B | zinc finger and SCAN domain... | XM_006723189.3 | 89.2% | 79% | (many diffs) |
| 18 | human | 342933 | ZSCAN5B | zinc finger and SCAN domain... | XM_005258864.3 | 80.6% | 71.4% | (many diffs) |
| 19 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322077.2 | 75.8% | 74.3% | (many diffs) |
| 20 | human | 79149 | ZSCAN5A | zinc finger and SCAN domain... | NM_001322078.2 | 72.7% | 70.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1554
- ORF length:
- 1488
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaaattgc acatcctcat ggagtctagg agaatcctgc aacagacctg 121 ggttggagct gccacggtct atggcatcct cagaaactca acttggaaat cacgacgtgg 181 accctgagat ttctcacgtg aacttcagga tgttcagctg cccgaaggag tcggacccca 241 tccaggctct gaggaaactc actgagctgt gccatctgtg gctgaggccc gacctccaca 301 ccaaagagca gatcctggac atgctggtga tggagcagtt catgatctcc atgccccagg 361 agctccaggt cttagtcatg atgaacggtg tgcagagctg caaagacctg gaggacctgc 421 tacgaaataa cagaagaccc aagaaatggt ctgtggtcac cttccacgga aaggaatata 481 ttgtgcagga ctcagatatc gagatggctg aagccccctc cagtgtcaga gatgatctga 541 aagacgtgtc cagccaacgg gcctcctcgg tgaaccagat gcgtccaggg gaaggccagg 601 cccaccgaga gctgcagatc ctgcccaggg tccctgcatt gtccaggagg cagggagagg 661 actttctgct acacaagagt attgacgtaa caggtgaccc aaagtctctg agacccaagc 721 agaccttgga gaaggatctg aaggaaaaca gggaagagaa cccaggactg acatccccag 781 agcctcagct tccaaagagt cccacagatc tggtgagagc aaaggagggg aaggaccccc 841 caaaaatagc ctctgtggaa aatgtggatg ctgacacacc ttctgcctgc gttgtggaga 901 gagaagcttc gactcacagc gggaacagag gagacgctct gaatctgagc agtcccaaaa 961 gaagcaaacc agatgcctcc tccatttccc aagaagagcc tcaaggagaa gccacacctg 1021 tgggcaacag agaatccccg ggacaagctg ggatgaattc aattcattcc ccaggccctg 1081 cgagcccagt cagtcacccg gatggccaag aagccaaggc actgccgccc tttgcatgtg 1141 acgtgtgcga gaagaggttt acgtgtaatt cCAAGCTAGT CATCCACAAG AGATCACACA 1201 CAGGCGAGAG ACTCTTTCAA TGTAATCTCT GTGGGAAGCG CTTCATGCAG CTTATTAGCC 1261 TCCAATTTCA CCAGCGAACC CACACTGGCG AGAGGCCCTA CACGTGTGAC GTCTGCCAGA 1321 AGCAGTTCAC CCAGAAGTCC TACTTGAAGT GTCACAAGAG AAGCCACACA GGGGAGAAGC 1381 CCTTCGAATG TAAAGACTGC AAGAAAGTTT TCACCTACAG GGGGAGCCTG AAGGAGCACC 1441 AGCGCATCCA CTCCGGAGAG AAACCCTACA AATGTTCCAA GTGTCCAAGA GCCTTCAGTC 1501 GGCTGAAATT GTTAAGACGC CACCAGAAAA CACATCCAGA AGCTACTTCT CAGTGCCCAA 1561 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1621 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1681 TATCTTGTGG AAAGGACGAC GCTGCCAATC CTCCGATCTC TAGACGCGTT AAGTCgacaa 1741 tcaacctctg gattacaaaa tttgtgaaag att