Transcript: Human NM_001322279.2

Homo sapiens methionyl aminopeptidase type 1D, mitochondrial (METAP1D), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
METAP1D (254042)
Length:
3099
CDS:
422..1075

Additional Resources:

NCBI RefSeq record:
NM_001322279.2
NBCI Gene record:
METAP1D (254042)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322279.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052160 CCACTCAATCATATCTACTTA pLKO.1 131 5UTR 100% 5.625 4.500 N METAP1D n/a
2 TRCN0000052158 CGGGAAATCATCAGTCATAAT pLKO.1 455 CDS 100% 13.200 9.240 N METAP1D n/a
3 TRCN0000429998 GGACATGGAATAGGATCTTAC pLKO_005 818 CDS 100% 10.800 7.560 N METAP1D n/a
4 TRCN0000052162 CATCATGCAAACGACAGTGAT pLKO.1 863 CDS 100% 4.950 3.465 N METAP1D n/a
5 TRCN0000052161 CTCTGTAATTGGAAACACAAT pLKO.1 748 CDS 100% 4.950 3.465 N METAP1D n/a
6 TRCN0000430391 ATGGAGATATTATCAACATTG pLKO_005 579 CDS 100% 10.800 6.480 N METAP1D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322279.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05297 pDONR223 100% 64.7% 64.7% None 0_1ins354 n/a
2 ccsbBroad304_05297 pLX_304 0% 64.7% 64.7% V5 0_1ins354 n/a
3 TRCN0000476162 GGTTCCATCTAAAGTGATAGATCC pLX_317 31.7% 64.7% 64.7% V5 0_1ins354 n/a
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