Construct: ORF TRCN0000476162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004482.1_s317c1
- Derived from:
- ccsbBroadEn_05297
- DNA Barcode:
- GGTTCCATCTAAAGTGATAGATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- METAP1D (254042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254042 | METAP1D | methionyl aminopeptidase ty... | NM_199227.3 | 100% | 100% | |
| 2 | human | 254042 | METAP1D | methionyl aminopeptidase ty... | XM_017003750.2 | 83.5% | 84.1% | (many diffs) |
| 3 | human | 254042 | METAP1D | methionyl aminopeptidase ty... | NM_001322278.2 | 64.7% | 64.7% | 0_1ins354 |
| 4 | human | 254042 | METAP1D | methionyl aminopeptidase ty... | NM_001322279.2 | 64.7% | 64.7% | 0_1ins354 |
| 5 | human | 254042 | METAP1D | methionyl aminopeptidase ty... | NR_136273.2 | 30% | (many diffs) | |
| 6 | human | 254042 | METAP1D | methionyl aminopeptidase ty... | NR_136276.2 | 28% | 1_17del;363_364ins149;874_2900del | |
| 7 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | NM_025633.3 | 90.4% | 91% | (many diffs) |
| 8 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319186.1 | 75.1% | 69.2% | (many diffs) |
| 9 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_011239738.2 | 70.9% | 64.8% | (many diffs) |
| 10 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XR_001783162.1 | 70.7% | (many diffs) | |
| 11 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319188.1 | 69.2% | 64.3% | (many diffs) |
| 12 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319189.1 | 69.2% | 64.3% | (many diffs) |
| 13 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XR_001783161.1 | 68.1% | (many diffs) | |
| 14 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319191.1 | 64.8% | 63.3% | (many diffs) |
| 15 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319190.1 | 64.6% | 62.9% | (many diffs) |
| 16 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319192.1 | 63.8% | 63.2% | (many diffs) |
| 17 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319187.1 | 63.5% | 62.2% | (many diffs) |
| 18 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_017319193.1 | 53.3% | 44% | (many diffs) |
| 19 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_006500008.2 | 49.4% | 45% | (many diffs) |
| 20 | mouse | 66559 | Metap1d | methionyl aminopeptidase ty... | XM_006500009.3 | 49.4% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1074
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgccc agtggcgtcc acctgctcgt ccgcagaggt tctcatagaa 121 ttttctcttc accactcaat catatctact tacacaagca gtcaagcagt caacaaagaa 181 gaaatttctt ttttcggaga caaagagata tttcacacag tatagttttg ccggctgcag 241 tttcttcagc tcatccggtt cctaagcaca taaagaagcc agactatgtg acgacaggca 301 ttgtaccaga ctggggagac agcatagaag ttaagaatga agatcagatt caagggcttc 361 atcaggcttg tcagctggcc cgccacgtcc tcctcttggc tgggaagagt ttaaaggttg 421 acatgacaac tgaagagata gatgctcttg ttcatcggga aatcatcagt cataatgcct 481 atccctcacc tctaggctat ggaggttttc caaaatctgt ttgtacctct gtaaacaacg 541 tgctctgtca tggtattcct gacagtcgac ctcttcagga tggagatatt atcaacattg 601 atgtcacagt ctattacaat ggctaccatg gagacacctc tgaaacattt ttggtgggca 661 atgtggacga atgtggtaaa aagttagtgg aggttgccag gaggtgtaga gatgaagcaa 721 ttgcagcttg cagagcaggg gctcccttct ctgtaattGG AAACACAATC AGCCACATAA 781 CTCATCAGAA TGGTTTTCAA GTCTGTCCAC ATTTTGTGGG ACATGGAATA GGATCTTACT 841 TTCATGGACA TCCAGAAATT TGGCATCATG CAAACGACAG TGATCTACCC ATGGAGGAGG 901 GCATGGCATT CACTATAGAG CCAATCATCA CGGAGGGATC CCCTGAATTT AAAGTCCTGG 961 AGGATGCATG GACTGTGGTC TCCCTAGACA ATCAAAGGTC GGCGCAGTTC GAGCACACGG 1021 TTCTGATCAC GTCGAGGGGC GCGCAGATCC TGACCAAACT ACCCCATGAG GCCTTGCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAG GTTCCATCTA AAGTGATAGA TCCACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att