Transcript: Human NM_001326418.2

Homo sapiens phosphatidylserine decarboxylase (PISD), transcript variant 10, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
PISD (23761)
Length:
2278
CDS:
108..1157

Additional Resources:

NCBI RefSeq record:
NM_001326418.2
NBCI Gene record:
PISD (23761)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001326418.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414132 CCGTTCCTTCTGCCTACAAAT pLKO_005 1369 3UTR 100% 13.200 18.480 N PISD n/a
2 TRCN0000416538 GAATGATCTCAGTCACATTTG pLKO_005 1405 3UTR 100% 10.800 8.640 N PISD n/a
3 TRCN0000078465 CAAGGGCTCCTACAATGACTT pLKO.1 962 CDS 100% 4.950 3.960 N PISD n/a
4 TRCN0000078466 GATGGAAGGATCCTCAACTTT pLKO.1 498 CDS 100% 5.625 3.938 N PISD n/a
5 TRCN0000078464 AGCCTGTACATCTGGACGTTT pLKO.1 348 CDS 100% 4.950 3.465 N PISD n/a
6 TRCN0000078463 CCCTACATGAAATATGGGAAA pLKO.1 1953 3UTR 100% 4.050 2.835 N PISD n/a
7 TRCN0000115413 CAGTATGAGAAGTACAGGGAA pLKO.1 171 CDS 100% 2.640 1.848 N Pisd n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001326418.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02840 pDONR223 100% 84.7% 76.6% None (many diffs) n/a
2 ccsbBroad304_02840 pLX_304 0% 84.7% 76.6% V5 (many diffs) n/a
3 TRCN0000470351 TCTTGCATCGTGTGAACGACTCAT pLX_317 44.3% 84.7% 76.6% V5 (many diffs) n/a
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