Transcript: Human NM_001348522.1

Homo sapiens KIAA1257 (KIAA1257), transcript variant 4, mRNA.

Source:
NCBI, updated 2018-12-22
Taxon:
Homo sapiens (human)
Gene:
KIAA1257 (57501)
Length:
1620
CDS:
438..1331

Additional Resources:

NCBI RefSeq record:
NM_001348522.1
NBCI Gene record:
KIAA1257 (57501)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001348522.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000268533 AGTCAGATATTACCGATTAAA pLKO_005 704 CDS 100% 15.000 21.000 N KIAA1257 n/a
2 TRCN0000268545 CGATTCTTCCACGATTCAATG pLKO_005 980 CDS 100% 10.800 15.120 N KIAA1257 n/a
3 TRCN0000268596 CACTGTGACAAAGGAATTATT pLKO_005 617 CDS 100% 15.000 12.000 N KIAA1257 n/a
4 TRCN0000268597 GCATTGAGAATACCAACATTG pLKO_005 814 CDS 100% 10.800 7.560 N KIAA1257 n/a
5 TRCN0000268532 CTACCTTGCCTGAGATCACAC pLKO_005 1453 3UTR 100% 4.050 2.430 N KIAA1257 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001348522.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14231 pDONR223 100% 99.8% 99.6% None 881C>A n/a
2 ccsbBroad304_14231 pLX_304 0% 99.8% 99.6% V5 881C>A n/a
3 TRCN0000472915 TAAAGCCAAGCTTTAGGTACCCTT pLX_317 34.6% 99.8% 99.6% V5 881C>A n/a
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