Construct: ORF TRCN0000472915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017259.1_s317c1
- Derived from:
- ccsbBroadEn_14231
- DNA Barcode:
- TAAAGCCAAGCTTTAGGTACCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA1257 (57501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57501 | KIAA1257 | KIAA1257 | NM_001348522.1 | 99.8% | 99.6% | 881C>A |
| 2 | human | 57501 | KIAA1257 | KIAA1257 | NM_001348523.1 | 99.8% | 99.6% | 881C>A |
| 3 | human | 57501 | KIAA1257 | KIAA1257 | NM_020741.3 | 72.5% | 72.3% | 1_336del;1217C>A |
| 4 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006948.2 | 27.4% | 27.4% | (many diffs) |
| 5 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006950.2 | 27.4% | 27.4% | (many diffs) |
| 6 | human | 57501 | KIAA1257 | KIAA1257 | XM_024453688.1 | 27.4% | 27.4% | (many diffs) |
| 7 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006946.2 | 26.2% | 26.2% | (many diffs) |
| 8 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006945.1 | 25.7% | 25.7% | (many diffs) |
| 9 | human | 57501 | KIAA1257 | KIAA1257 | XM_011513050.2 | 25% | 25% | (many diffs) |
| 10 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006944.2 | 24.7% | 24.7% | (many diffs) |
| 11 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006939.2 | 24.1% | 24% | (many diffs) |
| 12 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006940.2 | 24.1% | 24% | (many diffs) |
| 13 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006941.2 | 24.1% | 24% | (many diffs) |
| 14 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006942.2 | 24.1% | 24% | (many diffs) |
| 15 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006947.2 | 21.4% | 17.4% | (many diffs) |
| 16 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006951.2 | 19.2% | 19.2% | (many diffs) |
| 17 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006952.2 | 19.2% | 19.2% | (many diffs) |
| 18 | human | 57501 | KIAA1257 | KIAA1257 | XM_017006953.2 | 19.2% | 19.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tcgtttttac cacattgagt atttccttct gccggacgat gaagaaccta 121 aaaaagttga catattgcta tttccaatgg tggccaaagt attcctggag tcaggagtaa 181 agactgtgaa gccgtggcac gaaggtgaca aagcctgggt gtcgtgggag cagactttta 241 atatcactgt gacaaaggaa ttattaaaga aaataaattt ccacaaaatc accttgaggc 301 tctggaacac taaagacaag atgtcaagaa aagtcagata ttaccgatta aagactgccg 361 gcttcacaga cgacgtggga gcttttcata agtcagaagt gagacatttg gttttaaatc 421 agagaaaatt atctgaacag ggcattgaga ataccaacat tgtcagagaa gagtcgaacc 481 aggaacatcc gccaggaaaa caagaaaaaa cagaaaaaca cccaaagtct ttgcaaggtt 541 ctcaccaagc agagcccgaa acttcttcca agaacagtga ggaatatgag aagtccctca 601 aaatggacga ttcttccacg attcaatgga gtgtttcaag aacaccaacc aTTTCTTTGG 661 CAGGAGCAAG CATGATGGAG ATCAAGGAAT TAATTGAGAG TGAATCACTT AGCAGCTTAA 721 CAAACATATT AGACAGACAA AGGTCTCAAA TTAAAGGGAA AGATTCAGAG GGAAGAAGGA 781 AAATCCAGAG GAGACATAAG AAGCCCCTGG CAGAAGAAGA GGCAGACCCC ACGCTGACAG 841 GCCCCAGGAA GCAGAGCGCT TTCTCCATCC AGCTGGCCGT CATGCCGCTC CTTGCCGGTA 901 CCCACTGCTT GCCCTGTTCC CAGCAGCTCC TGCTTGTCTT GTGGCAAGAA CGGCCATACC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATAAAGCCA AGCTTTAGGT ACCCTTACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt