Construct: ORF ccsbBroad304_06671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007681.1_s304c1, BRDN0000462369
- Derived from:
- ccsbBroadEn_06671
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPA1 (4976)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06671
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_015560.2 | 99.9% | 99.8% | 473G>A;2109T>C |
| 2 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130834.3 | 98% | 98% | 473G>A;555_608del;2163T>C |
| 3 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130832.3 | 97.2% | 96.5% | (many diffs) |
| 4 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130836.3 | 96.2% | 96.1% | 473G>A;622_732del;2220T>C |
| 5 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130831.3 | 96.2% | 96.2% | 447_448ins108;2001T>C |
| 6 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130837.2 | 94.5% | 94.4% | (many diffs) |
| 7 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130835.2 | 93.6% | 92.9% | (many diffs) |
| 8 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_130833.2 | 92.6% | 92.6% | 447_448ins108;514_624del;2112T>C |
| 9 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_001354664.2 | 87% | 86.9% | 0_1ins372;101G>A;1737T>C |
| 10 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | NM_001354663.2 | 80.2% | 80.2% | (many diffs) |
| 11 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | XR_001740159.2 | 45.3% | (many diffs) | |
| 12 | human | 4976 | OPA1 | OPA1 mitochondrial dynamin ... | XR_001740158.2 | 44.1% | (many diffs) | |
| 13 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | NM_133752.3 | 88.5% | 96.2% | (many diffs) |
| 14 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | NM_001199177.1 | 87% | 94.4% | (many diffs) |
| 15 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | XM_006522657.3 | 78.6% | 85.3% | (many diffs) |
| 16 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | XM_030249307.1 | 78.6% | 85.3% | (many diffs) |
| 17 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | XM_006522654.4 | 77.4% | 84% | (many diffs) |
| 18 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | XM_006522655.3 | 77.4% | 84% | (many diffs) |
| 19 | mouse | 74143 | Opa1 | OPA1, mitochondrial dynamin... | XM_006522656.4 | 77.4% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2949
- ORF length:
- 2880
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggcgacta cgtcgggccg ctgtggcctg tgaggtctgc cagtctttag 121 tgaaacacag ctctggaata aaaggaagtt taccactaca aaaactacat ctggtttcac 181 gaagcattta tcattcacat catcctacct taaagcttca acgaccccaa ttaaggacat 241 cctttcagca gttctcttct ctgacaaacc ttcctttacg taaactgaaa ttctctccaa 301 ttaaatatgg ctaccagcct cgcaggaatt tttggccagc aagattagct acgagactct 361 taaaacttcg ctatctcata ctaggatcgg ctgttggggg tggctacaca gccaaaaaga 421 cttttgatca gtggaaagat atgataccgg accttagtga atataaatgg attgtgcctg 481 acattgtgtg ggaaattgat gagtatatcg attttgagaa aattagaaaa gcccttccta 541 attcagaaga ccttgtaaag ttagcaccag actttgacaa gattgttgaa agccttagct 601 tattgaagga cttttttacc tcaggttctc cggaagaaac ggcgtttaga gcaacagatc 661 gtggatctga aagtgacaag cattttagaa aggtgtcaga caaagagaaa attgaccaac 721 ttcaggaaga acttctgcac actcagttga agtatcagag aatcttggaa cgattagaaa 781 aggagaacaa agaattgaga aaattagtat tgcagaaaga tgacaaaggc attcatcata 841 gaaagcttaa gaaatctttg attgacatgt attctgaagt tcttgatgtt ctctctgatt 901 atgatgccag ttataatacg caagatcatc tgccacgggt tgttgtggtt ggagatcaga 961 gtgctggaaa gactagtgtg ttggaaatga ttgcccaagc tcgaatattc ccaagaggat 1021 ctggggagat gatgacacgt tctccagtta aggtgactct gagtgaaggt cctcaccatg 1081 tggccctatt taaagatagt tctcgggagt ttgatcttac caaagaagaa gatcttgcag 1141 cattaagaca tgaaatagaa cttcgaatga ggaaaaatgt gaaagaaggc tgtaccgtta 1201 gccctgagac catatcctta aatgtaaaag gccctggact acagaggatg gtgcttgttg 1261 acttaccagg tgtgattaat actgtgacat caggcatggc tcctgacaca aaggaaacta 1321 ttttcagtat cagcaaagct tacatgcaga atcctaatgc catcatactg tgtattcaag 1381 atggatctgt ggatgctgaa cgcagtattg ttacagactt ggtcagtcaa atggaccctc 1441 atggaaggag aaccatattc gttttgacca aagtagacct ggcagagaaa aatgtagcca 1501 gtccaagcag gattcagcag ataattgaag gaaagctctt cccaatgaaa gctttaggtt 1561 attttgctgt tgtaacagga aaagggaaca gctctgaaag cattgaagct ataagagaat 1621 atgaagaaga gttttttcag aattcaaagc tcctaaagac aagcatgcta aaggcacacc 1681 aagtgactac aagaaattta agccttgcag tatcagactg cttttggaaa atggtacgag 1741 agtctgttga acaacaggct gatagtttca aagcaacacg ttttaacctt gaaactgaat 1801 ggaagaataa ctatcctcgc ctgcgggaac ttgaccggaa tgaactattt gaaaaagcta 1861 aaaatgaaat ccttgatgaa gttatcagtc tgagccaggt tacaccaaaa cattgggagg 1921 aaatccttca acaatctttg tgggaaagag tatcaactca tgtgattgaa aacatctacc 1981 ttccagctgc gcagaccatg aattcaggaa cttttaacac cacagtggat atcaagctta 2041 aacagtggac tgataaacaa cttcctaata aagcagtaga ggttgcttgg gagaccctac 2101 aagaagaatt ttcccgcttt atgacagaac cgaaagggaa agagcatgat gacatatttg 2161 ataaacttaa agaggccgtt aaggaagaaa gtattaaacg acacaagtgg aatgactttg 2221 cggaggacag cttgagggtt attcaacaca atgctttgga agaccgatcc atatctgata 2281 aacagcaatg ggatgcagct atttatttta tggaagaggc tctgcaggct cgtctcaagg 2341 atactgaaaa tgcaattgaa aacatggtgg gtccagactg gaaaaagagg tggttatact 2401 ggaagaatcg gacccaagaa cagtgtgttc acaatgaaac caagaatgaa ttggagaaga 2461 tgttgaaatg taatgaggag cacccagctt atcttgcaag tgatgaaata accacagtcc 2521 ggaagaacct tgaatcccga ggagtagaag tagatccaag cttgattaag gatacttggc 2581 atcaagttta tagaagacat tttttaaaaa cagctctaaa ccattgtaac ctttgtcgaa 2641 gaggttttta ttactaccaa aggcattttg tagattctga gttggaatgc aatgatgtgg 2701 tcttgttttg gcgtatacag cgcatgcttg ctatcaccgc aaatacttta aggcaacaac 2761 ttacaaatac tgaagttagg cgattagaga aaaatgttaa agaggtattg gaagattttg 2821 ctgaagatgg tgagaagaag attaaattgc ttactggtaa acgcgttcaa ctggcggaag 2881 acctcaagaa agttagagaa attcaagaaa aacttgatgc tttcattgaa gctcttcatc 2941 aggagaaatt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 3001 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 3061 tcaacctctg gatta