Transcript: Human NM_001372043.1

Homo sapiens proprotein convertase subtilisin/kexin type 5 (PCSK5), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
PCSK5 (5125)
Length:
10226
CDS:
510..6173

Additional Resources:

NCBI RefSeq record:
NM_001372043.1
NBCI Gene record:
PCSK5 (5125)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001372043.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000222011 GCGGGACATTTGAACGCTAAT pLKO.1 1770 CDS 100% 10.800 15.120 N Pcsk5 n/a
2 TRCN0000051182 GCCCAGTCTACCTATTTCAAT pLKO.1 870 CDS 100% 5.625 7.875 N PCSK5 n/a
3 TRCN0000287024 GCCCAGTCTACCTATTTCAAT pLKO_005 870 CDS 100% 5.625 7.875 N PCSK5 n/a
4 TRCN0000051179 CCAACCAATGAATTTCCGAAA pLKO.1 2322 CDS 100% 4.050 5.670 N PCSK5 n/a
5 TRCN0000294471 TACCATAGCAGGACGATTAAA pLKO_005 732 CDS 100% 0.000 0.000 N PCSK5 n/a
6 TRCN0000294470 ACTATGGCACAGAGGATTATG pLKO_005 2386 CDS 100% 13.200 9.240 N PCSK5 n/a
7 TRCN0000051180 CCTGCCAGTCTGACATGAATA pLKO.1 943 CDS 100% 13.200 9.240 N PCSK5 n/a
8 TRCN0000287084 CCTGCCAGTCTGACATGAATA pLKO_005 943 CDS 100% 13.200 9.240 N PCSK5 n/a
9 TRCN0000051178 CCTGTGAAGATGGACGGTATT pLKO.1 2818 CDS 100% 10.800 7.560 N PCSK5 n/a
10 TRCN0000287083 CCTGTGAAGATGGACGGTATT pLKO_005 2818 CDS 100% 10.800 7.560 N PCSK5 n/a
11 TRCN0000051181 GCTATTACTTTGACCACTCTT pLKO.1 2971 CDS 100% 4.950 3.465 N PCSK5 n/a
12 TRCN0000222013 CCTCGTTATGATGCAAGCAAT pLKO.1 1113 CDS 100% 4.950 6.930 N Pcsk5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001372043.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06697 pDONR223 100% 47.7% 46.7% None (many diffs) n/a
2 ccsbBroad304_06697 pLX_304 0% 47.7% 46.7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000468891 TGGTCCTTATAGGCAGCTTCCTAG pLX_317 .7% 47.7% 46.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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