Construct: ORF TRCN0000468891
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002475.1_s317c1
- Derived from:
- ccsbBroadEn_06697
- DNA Barcode:
- TGGTCCTTATAGGCAGCTTCCTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PCSK5 (5125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468891
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5125 | PCSK5 | proprotein convertase subti... | NM_006200.6 | 99.9% | 99.7% | 2734C>T |
| 2 | human | 5125 | PCSK5 | proprotein convertase subti... | XR_929807.2 | 77.1% | (many diffs) | |
| 3 | human | 5125 | PCSK5 | proprotein convertase subti... | NM_001190482.1 | 48.4% | 47.4% | (many diffs) |
| 4 | human | 5125 | PCSK5 | proprotein convertase subti... | XM_011518769.3 | 47.7% | 46.7% | (many diffs) |
| 5 | human | 5125 | PCSK5 | proprotein convertase subti... | NM_001372043.1 | 47.7% | 46.7% | (many diffs) |
| 6 | human | 5125 | PCSK5 | proprotein convertase subti... | XR_929806.2 | 45.6% | (many diffs) | |
| 7 | human | 5125 | PCSK5 | proprotein convertase subti... | NR_120409.2 | 45.5% | (many diffs) | |
| 8 | human | 5125 | PCSK5 | proprotein convertase subti... | XM_017014800.1 | 24.5% | 23.5% | (many diffs) |
| 9 | human | 5125 | PCSK5 | proprotein convertase subti... | XM_011518770.2 | 24.1% | 23.1% | (many diffs) |
| 10 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | NM_001163144.1 | 88.2% | 94.5% | (many diffs) |
| 11 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | XM_006526761.3 | 76.2% | 78.2% | (many diffs) |
| 12 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | XM_006526760.2 | 64.8% | 66.6% | (many diffs) |
| 13 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | XM_006526759.3 | 43.3% | 44.7% | (many diffs) |
| 14 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | XM_011247162.2 | 42.7% | 44.1% | (many diffs) |
| 15 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | NM_001190483.2 | 42.7% | 44% | (many diffs) |
| 16 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | XR_386446.3 | 42.3% | (many diffs) | |
| 17 | mouse | 18552 | Pcsk5 | proprotein convertase subti... | XM_017318080.1 | 36.4% | 37.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2799
- ORF length:
- 2733
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgggggagc cgctgctgct gcccgggacg tttggacctg ctgtgcgtgc 121 tggcgctgct cgggggctgc ctgctccccg tgtgtcggac gcgcgtctac accaaccact 181 gggcagtcaa aatcgccggg ggcttcccgg aggccaaccg tatcgccagc aagtacggat 241 tcatcaacat aggacagata ggggccctga aggactacta ccacttctac catagcagga 301 cgattaaaag gtcagttatc tcgagcagag ggacccacag tttcatttca atggaaccaa 361 aggtggaatg gatccaacag caagtggtaa aaaagcggac aaagagggat tatgacttca 421 gtcgtgccca gtctacctat ttcaatgatc ccaagtggcc cagcatgtgg tatatgcact 481 gcagtgacaa tacacatccc tgccagtctg acatgaatat cgaaggagcc tggaagagag 541 gctacacggg aaagaacatt gtggtcacta tcctggatga cggaattgag agaacccatc 601 cagatctgat gcaaaactac gatgctctgg caagttgcga cgtgaatggg aatgacttgg 661 acccaatgcc tcgttatgat gcaagcaacg agaacaagca tgggactcgc tgtgctggag 721 aagtggcagc cgctgcaaac aattcgcact gcacagtcgg aattgctttc aacgccaaga 781 tcggaggagt gcgaatgctg gacggagatg tcacggacat ggttgaagca aaatcagtta 841 gcttcaaccc ccagcacgtg cacatttaca gcgccagctg gggcccggat gatgatggca 901 agactgtgga cggaccagcc cccctcaccc ggcaagcctt tgaaaacggc gttagaatgg 961 ggcggagagg cctcggctct gtgtttgttt gggcatctgg aaatggtgga aggagcaaag 1021 accactgctc ctgtgatggc tacaccaaca gcatctacac catctccatc agcagcactg 1081 cagaaagcgg aaagaaacct tggtacctgg aagagtgttc atccacgctg gccacaacct 1141 acagcagcgg ggagtcctac gataagaaaa tcatcactac agatctgagg cagcgttgca 1201 cggacaacca cactgggacg tcagcctcag cccccatggc tgcaggcatc attgcgctgg 1261 ccctggaagc caatccgttt ctgacctgga gagacgtaca gcatgttatt gtcaggactt 1321 cccgtgcggg acatttgaac gctaatgact ggaaaaccaa tgctgctggt tttaaggtga 1381 gccatcttta tggatttgga ctgatggacg cagaagccat ggtgatggag gcagagaagt 1441 ggaccaccgt tccccggcag cacgtgtgtg tggagagcac agaccgacaa atcaagacaa 1501 tccgccctaa cagtgcagtg cgctccatct acaaagcttc aggctgctcg gataacccca 1561 accgccatgt caactacctg gagcacgtcg ttgtgcgcat caccatcacc caccccagga 1621 gaggagacct ggccatctac ctgacctcgc cctctggaac taggtctcag cttttggcca 1681 acaggctatt tgatcactcc atggaaggat tcaaaaactg ggagttcatg accattcatt 1741 gctggggaga aagagctgct ggtgactggg tccttgaagt ttatgatact ccctctcagc 1801 taaggaactt taagactcca ggtaaattga aagaatggtc tttggtcctc tacggcacct 1861 ccgtgcagcc atattcacca accaatgaat ttccgaaagt ggaacggttc cgctatagcc 1921 gagttgaaga ccccacagac gactatggca cagaggatta tgcaggtccc tgcgaccctg 1981 agtgcagtga ggttggctgt gacgggccag gaccagacca ctgcaatgac tgtttgcact 2041 actactacaa gctgaaaaac aataccagga tctgtgtctc cagctgcccc cctggccact 2101 accacgccga caagaagcgc tgcaggaagt gtgcccccaa ctgtgagtcc tgctttggga 2161 gccatggtga ccaatgcatg tcctgcaaat atggatactt tctgaatgaa gaaaccaaca 2221 gctgtgttac tcactgccct gatgggtcat atcaggatac caagaaaaat ctttgccgga 2281 aatgcagtga aaactgcaag acatgtactg aattccataa ctgtacagaa tgtagggatg 2341 ggttaagcct gcagggatcc cggtgctctg tctcctgtga agatggacgg tatttcaacg 2401 gccaggactg ccagccctgc caccgcttct gcgccacttg tgctggggca ggagctgatg 2461 ggtgcattaa ctgcacagag ggctacttca tggaggatgg gagatgcgtg cagagctgta 2521 gtatcagcta ttactttgac cactcttcag agaatggata caaatcctgc aaaaaatgtg 2581 atatcagttg tttgacgtgc aatggcccag gattcaagaa ctgtacaagc tgccctagtg 2641 ggtatctctt agacttagga atgtgtcaaa tgggagccat ttgcaaggat gcaacggaag 2701 agtcctgggc ggaaggaggc ttctgtatgc ttgtgaaaaa gaacaatctg tgccaacgga 2761 aggttcttca acaactttgc tgcaaaacat gtaCATTTTA AGGCTGCCCA ACTTTCTTGT 2821 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2881 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2941 GAAAGGACGA TGGTCCTTAT AGGCAGCTTC CTAGACGCGT TAAGTCgaca atcaacctct 3001 ggattacaaa atttgtgaaa gatt