Transcript: Human NM_003328.3

Homo sapiens TXK tyrosine kinase (TXK), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
TXK (7294)
Length:
2908
CDS:
81..1664

Additional Resources:

NCBI RefSeq record:
NM_003328.3
NBCI Gene record:
TXK (7294)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145857 AGAACATCTATTGAGACAAG pXPR_003 AGG 496 31% 6 0.6442 TXK TXK 76358
2 BRDN0001146212 TGGTCCATTTAGGTGAATGG pXPR_003 CGG 867 55% 10 0.2586 TXK TXK 76360
3 BRDN0001145866 GGAAGGCAAGAGACCGTTTG pXPR_003 GGG 375 24% 4 -0.2161 TXK TXK 76359
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003328.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121108 CCTACACAATTTCCGTATTTA pLKO.1 628 CDS 100% 15.000 21.000 N TXK n/a
2 TRCN0000121181 GAGTCTAAAGAAGGTGCATTT pLKO.1 579 CDS 100% 10.800 15.120 N TXK n/a
3 TRCN0000121180 GCACCAATGTCCATATATGAA pLKO.1 1551 CDS 100% 5.625 7.875 N TXK n/a
4 TRCN0000195084 CAAGGAATTGTTTGGTCAGTT pLKO.1 1258 CDS 100% 4.950 6.930 N TXK n/a
5 TRCN0000009998 CCGTATTTATGGGAGCTAGAA pLKO.1 640 CDS 100% 4.950 6.930 N TXK n/a
6 TRCN0000055429 CCGTATTTATGGGAGCTAGAA pLKO.1 640 CDS 100% 4.950 6.930 N TXK n/a
7 TRCN0000121111 CCTTTACATTGTGACAGAGTT pLKO.1 1094 CDS 100% 4.950 6.930 N TXK n/a
8 TRCN0000009999 GACACGCCTTTCAATCAATCC pLKO.1 733 CDS 100% 4.050 5.670 N TXK n/a
9 TRCN0000001578 GCGACAAATGAGAACACAGAT pLKO.1 149 CDS 100% 4.950 6.435 N TXK n/a
10 TRCN0000196957 GCAAGTCGTGGAAGCTATTTC pLKO.1 1499 CDS 100% 13.200 10.560 N TXK n/a
11 TRCN0000121110 GCCCAGAGAACCCTGTAATTT pLKO.1 362 CDS 100% 15.000 10.500 N TXK n/a
12 TRCN0000196460 GCTCAGCCAATTGTCAAATAA pLKO.1 227 CDS 100% 15.000 10.500 N TXK n/a
13 TRCN0000194951 CAGTGATCCATGCATAGAATA pLKO.1 2044 3UTR 100% 13.200 9.240 N TXK n/a
14 TRCN0000194938 CAGTTCAACATGCATAGTAAA pLKO.1 1274 CDS 100% 13.200 9.240 N TXK n/a
15 TRCN0000314836 CAGTTCAACATGCATAGTAAA pLKO_005 1274 CDS 100% 13.200 9.240 N TXK n/a
16 TRCN0000121107 CCCTTGAAGAATTTCATGTTT pLKO.1 2104 3UTR 100% 5.625 3.938 N TXK n/a
17 TRCN0000194674 CCTGAGTTAATCTGGTATCAC pLKO.1 753 CDS 100% 4.950 3.465 N TXK n/a
18 TRCN0000196365 GCACCTTTATAAATCACATGA pLKO.1 2153 3UTR 100% 4.950 3.465 N TXK n/a
19 TRCN0000314838 GCACCTTTATAAATCACATGA pLKO_005 2153 3UTR 100% 4.950 3.465 N TXK n/a
20 TRCN0000121177 GCCAACCCAAAGAGTCATCTT pLKO.1 1678 3UTR 100% 4.950 3.465 N TXK n/a
21 TRCN0000314784 GCCAACCCAAAGAGTCATCTT pLKO_005 1678 3UTR 100% 4.950 3.465 N TXK n/a
22 TRCN0000121288 GTCCATATATGAAGTCATGTA pLKO.1 1559 CDS 100% 4.950 3.465 N TXK n/a
23 TRCN0000001577 GAAACAGAATGCCAACCCAAA pLKO.1 1668 3UTR 100% 4.050 2.835 N TXK n/a
24 TRCN0000314837 GAAACAGAATGCCAACCCAAA pLKO_005 1668 3UTR 100% 4.050 2.835 N TXK n/a
25 TRCN0000121109 GCTTAACTATCTCAGGGAGAA pLKO.1 1133 CDS 100% 4.050 2.835 N TXK n/a
26 TRCN0000121291 TGCTGGCATGAGAAACCTGAA pLKO.1 1584 CDS 100% 4.050 2.835 N TXK n/a
27 TRCN0000001580 GCTGCCTGCTTAACTATCTCA pLKO.1 1126 CDS 100% 3.000 2.100 N TXK n/a
28 TRCN0000314783 GCTGCCTGCTTAACTATCTCA pLKO_005 1126 CDS 100% 3.000 2.100 N TXK n/a
29 TRCN0000121290 GTCAAATAAGAAGCAATCCAA pLKO.1 239 CDS 100% 3.000 2.100 N TXK n/a
30 TRCN0000121179 GCTGTCACAGAGATTGCGGAA pLKO.1 1635 CDS 100% 2.160 1.512 N TXK n/a
31 TRCN0000009997 TACCTGATACTGGAGAAATAC pLKO.1 405 CDS 100% 0.000 0.000 N TXK n/a
32 TRCN0000121178 CCAGGATATATGTGAAGGAAT pLKO.1 1193 CDS 100% 4.950 2.970 N TXK n/a
33 TRCN0000121287 CCCAAATGTGAAGAAGTGAAA pLKO.1 1902 3UTR 100% 4.950 2.970 N TXK n/a
34 TRCN0000001579 GAATACCTGATACTGGAGAAA pLKO.1 402 CDS 100% 4.950 2.970 N TXK n/a
35 TRCN0000196461 GAAGAGAAGATCCAAGTCAAG pLKO.1 324 CDS 100% 4.050 2.430 N TXK n/a
36 TRCN0000121289 GAGTGGTACCATAGAAACATT pLKO.1 525 CDS 100% 0.000 0.000 N TXK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003328.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14869 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_14869 pLX_304 0% 100% 100% V5 n/a
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