Transcript: Human NM_003827.4

Homo sapiens NSF attachment protein alpha (NAPA), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
NAPA (8775)
Length:
1662
CDS:
124..1011

Additional Resources:

NCBI RefSeq record:
NM_003827.4
NBCI Gene record:
NAPA (8775)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003827.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380024 CAAGAGGCCATTAACTGTTTG pLKO_005 415 CDS 100% 10.800 15.120 N NAPA n/a
2 TRCN0000381191 ACATGGGCCGATTCACGATTG pLKO_005 461 CDS 100% 6.000 8.400 N NAPA n/a
3 TRCN0000029170 CAGCGCCAAAGACTACTTCTT pLKO.1 723 CDS 100% 4.950 6.930 N NAPA n/a
4 TRCN0000293265 CAGCGCCAAAGACTACTTCTT pLKO_005 723 CDS 100% 4.950 6.930 N NAPA n/a
5 TRCN0000382195 TGAGTCATGAGGCCGGTTTCT pLKO_005 1362 3UTR 100% 4.950 6.930 N NAPA n/a
6 TRCN0000380266 TGCGAGCAATCGAGATCTACA pLKO_005 437 CDS 100% 4.950 6.930 N NAPA n/a
7 TRCN0000293268 TCTTTGATACAGACCTATTTG pLKO_005 1284 3UTR 100% 13.200 9.240 N NAPA n/a
8 TRCN0000293267 ATAGAGGAAGCATGCGAAATC pLKO_005 235 CDS 100% 10.800 7.560 N NAPA n/a
9 TRCN0000029171 GCTGGCAACGCATTCAAGAAA pLKO.1 385 CDS 100% 5.625 3.938 N NAPA n/a
10 TRCN0000382210 CACCCTCTGTTCTCGCTACAT pLKO_005 1194 3UTR 100% 4.950 3.465 N NAPA n/a
11 TRCN0000029169 CCGGGAATGCAAGTTGATGAA pLKO.1 837 CDS 100% 4.950 3.465 N NAPA n/a
12 TRCN0000293266 CCGGGAATGCAAGTTGATGAA pLKO_005 837 CDS 100% 4.950 3.465 N NAPA n/a
13 TRCN0000111543 CGCCAAAGACTACTTCTTCAA pLKO.1 726 CDS 100% 4.950 3.465 N Napa n/a
14 TRCN0000298396 CGCCAAAGACTACTTCTTCAA pLKO_005 726 CDS 100% 4.950 3.465 N Napa n/a
15 TRCN0000029173 GTATCAGAAGGCCATTGACAT pLKO.1 654 CDS 100% 4.950 3.465 N NAPA n/a
16 TRCN0000293264 GTATCAGAAGGCCATTGACAT pLKO_005 654 CDS 100% 4.950 3.465 N NAPA n/a
17 TRCN0000029172 CTGGCCTCTTTGGAGGCTCAT pLKO.1 209 CDS 100% 0.135 0.095 N NAPA n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003827.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02014 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02014 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472853 CTGTAGACAATTTAGACTTCCTCG pLX_317 46.2% 100% 100% V5 n/a
Download CSV