Transcript: Human NM_004931.5

Homo sapiens CD8b molecule (CD8B), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
CD8B (926)
Length:
4794
CDS:
22..654

Additional Resources:

NCBI RefSeq record:
NM_004931.5
NBCI Gene record:
CD8B (926)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004931.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000057511 CCTCAGTAACATGCGCATCTA pLKO.1 159 CDS 100% 4.950 2.970 N CD8B n/a
2 TRCN0000057510 GCTTCGTTTCATGAAACAATT pLKO.1 624 CDS 100% 13.200 6.600 Y CD8B n/a
3 TRCN0000371762 TCAGCTGAGTGTGGTTGATTT pLKO_005 411 CDS 100% 13.200 6.600 Y CD8B n/a
4 TRCN0000371712 CCTGGTAGGGCAGTAACATTG pLKO_005 1011 3UTR 100% 10.800 5.400 Y CD8B n/a
5 TRCN0000166498 CGCCTGTAATCCCAGTACTTT pLKO.1 4179 3UTR 100% 5.625 2.813 Y MGC13053 n/a
6 TRCN0000057508 GCTGGAGGACTGAGTAAGAAA pLKO.1 895 3UTR 100% 5.625 2.813 Y CD8B n/a
7 TRCN0000057512 GCAAGCCGGTTCATTCTCAAT pLKO.1 307 CDS 100% 4.950 2.475 Y CD8B n/a
8 TRCN0000180418 GATCACTTGAGCTCAGGAGTT pLKO.1 4218 3UTR 100% 4.050 2.025 Y ERN2 n/a
9 TRCN0000057509 CCTGCATACATAAAGGTGCAA pLKO.1 97 CDS 100% 2.640 1.320 Y CD8B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004931.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05956 pDONR223 100% 85.7% 85.1% None (many diffs) n/a
2 ccsbBroad304_05956 pLX_304 0% 85.7% 85.1% V5 (many diffs) n/a
3 TRCN0000466988 TTTGATACTTCGTTTTAAGAATTT pLX_317 48.5% 85.7% 85.1% V5 (many diffs) n/a
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