Construct: ORF TRCN0000466988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007705.1_s317c1
- Derived from:
- ccsbBroadEn_05956
- DNA Barcode:
- TTTGATACTTCGTTTTAAGAATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD8B (926)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 926 | CD8B | CD8b molecule | NM_172213.4 | 99.8% | 100% | 25T>C |
| 2 | human | 926 | CD8B | CD8b molecule | XM_011533164.2 | 87.9% | 85.3% | (many diffs) |
| 3 | human | 926 | CD8B | CD8b molecule | NM_172101.4 | 87.7% | 86% | (many diffs) |
| 4 | human | 926 | CD8B | CD8b molecule | NM_172102.4 | 87.5% | 87.6% | 25T>C;493_494ins90 |
| 5 | human | 927 | CD8B2 | CD8b2 molecule | XM_024453217.1 | 87.1% | 83.7% | (many diffs) |
| 6 | human | 926 | CD8B | CD8b molecule | NM_004931.5 | 85.7% | 85.1% | (many diffs) |
| 7 | human | 927 | CD8B2 | CD8b2 molecule | NM_001368307.2 | 85.3% | 84.3% | (many diffs) |
| 8 | human | 927 | CD8B2 | CD8b2 molecule | NM_001349727.2 | 84.9% | 83.5% | (many diffs) |
| 9 | human | 926 | CD8B | CD8b molecule | NM_001178100.1 | 81.3% | 65.9% | 25T>C;493_494ins127;594_595insTGAAAACA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 798
- ORF length:
- 729
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggccgcgg ctgtggctcc tcctggccgc gcagctgaca gttctccatg 121 gcaactcagt cctccagcag acccctgcat acataaaggt gcaaaccaac aagatggtga 181 tgctgtcctg cgaggctaaa atctccctca gtaacatgcg catctactgg ctgagacagc 241 gccaggcacc gagcagtgac agtcaccacg agttcctggc cctctgggat tccgcaaaag 301 ggactatcca cggtgaagag gtggaacagg agaagatagc tgtgtttcgg gatgcaagcc 361 ggttcattct caatctcaca agcgtgaagc cggaagacag tggcatctac ttctgcatga 421 tcgtcgggag ccccgagctg accTTCGGGA AGGGAACTCA GCTGAGTGTG GTTGATTTCC 481 TTCCCACCAC TGCCCAGCCC ACCAAGAAGT CCACCCTCAA GAAGAGAGTG TGCCGGTTAC 541 CCAGGCCAGA GACCCAGAAG GGCCCACTTT GTAGCCCCAT CACCCTTGGC CTGCTGGTGG 601 CTGGCGTCCT GGTTCTGCTG GTTTCCCTGG GAGTGGCCAT CCACCTGTGC TGCCGGCGGA 661 GGAGAGCCCG GCTTCGTTTC ATGAAACAGC CTCAAGGGGA AGGTATATCA GGAACCTTTG 721 TCCCCCAATG CCTGCATGGA TACTACAGCA ATACTACAAC CTCACAGAAG CTGCTTAACC 781 CATGGATCCT GAAAACATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTTGATA CTTCGTTTTA 961 AGAATTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt