Transcript: Human NM_005103.5

Homo sapiens fasciculation and elongation protein zeta 1 (FEZ1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
FEZ1 (9638)
Length:
4583
CDS:
191..1369

Additional Resources:

NCBI RefSeq record:
NM_005103.5
NBCI Gene record:
FEZ1 (9638)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005103.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000330402 TATAGCTTAACCTGGATTAAA pLKO_005 1475 3UTR 100% 15.000 21.000 N FEZ1 n/a
2 TRCN0000330331 TCCATGGAGGACCTCGTAAAT pLKO_005 362 CDS 100% 13.200 18.480 N FEZ1 n/a
3 TRCN0000330404 TCCTTTATCACGGTGCTTATT pLKO_005 995 CDS 100% 13.200 18.480 N FEZ1 n/a
4 TRCN0000062678 CCCAGTGTTTCTATGGTTCAT pLKO.1 270 CDS 100% 4.950 6.930 N FEZ1 n/a
5 TRCN0000062679 GCCTACTTTGCTAACGGACTA pLKO.1 1321 CDS 100% 4.050 5.670 N FEZ1 n/a
6 TRCN0000353602 CTCACAGCAGATCAGGTAATT pLKO_005 674 CDS 100% 13.200 9.240 N FEZ1 n/a
7 TRCN0000330403 TGGAAGGCATCTCCAACATTC pLKO_005 1140 CDS 100% 10.800 7.560 N FEZ1 n/a
8 TRCN0000062680 CCAAACATCGAGGCTCTGAAT pLKO.1 560 CDS 100% 4.950 3.465 N FEZ1 n/a
9 TRCN0000062682 GCTCAATGTCTGCTTTCGGAA pLKO.1 397 CDS 100% 2.640 1.848 N FEZ1 n/a
10 TRCN0000062681 CTCCTTTATCACGGTGCTTAT pLKO.1 994 CDS 100% 10.800 6.480 N FEZ1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005103.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02217 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02217 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000465310 GCACACGTCCTCGGGAGCTTGCGT pLX_317 33.6% 100% 100% V5 n/a
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