Transcript: Human NM_005198.4

Homo sapiens choline kinase beta (CHKB), mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
CHKB (1120)
Length:
1648
CDS:
219..1406

Additional Resources:

NCBI RefSeq record:
NM_005198.4
NBCI Gene record:
CHKB (1120)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148182 GCAGAAGACGACTGGCGATG pXPR_003 GGG 700 59% 6 0.7997 CHKB, CHKB-CPT1B CHKB 76866
2 BRDN0001147856 TGTTCGCCATACTTGCGGAG pXPR_003 CGG 381 32% 3 0.3753 CHKB, CHKB-CPT1B CHKB 76865
3 BRDN0001149487 AGCTGAGGGTTTACCCCGTG pXPR_003 AGG 219 18% 1 -0.1283 CHKB, CHKB-CPT1B CHKB 76864
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005198.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024966 GTGAGTGGGTTTATGATTATA pLKO.1 1063 CDS 100% 15.000 7.500 Y Chkb n/a
2 TRCN0000274563 GTGAGTGGGTTTATGATTATA pLKO_005 1063 CDS 100% 15.000 7.500 Y Chkb n/a
3 TRCN0000052555 GCCACGAAGATGGCGCAATTT pLKO.1 720 CDS 100% 13.200 6.600 Y CHKB n/a
4 TRCN0000052557 GCATCCATGTCCACCATAGAA pLKO.1 1299 CDS 100% 5.625 2.813 Y CHKB n/a
5 TRCN0000196694 GAAGAAGATTTGCTGGTAGAA pLKO.1 1221 CDS 100% 4.950 2.475 Y CHKB n/a
6 TRCN0000052553 GCTTTGACATTGGGAACCATT pLKO.1 1039 CDS 100% 4.950 2.475 Y CHKB n/a
7 TRCN0000052554 CCAGTTCTACTTCCAGCAGAA pLKO.1 1352 CDS 100% 4.050 2.025 Y CHKB n/a
8 TRCN0000195231 CCATAGAATTTGGTTACTTGG pLKO.1 1312 CDS 100% 4.050 2.025 Y CHKB n/a
9 TRCN0000199249 CCTCAGGAAGTTACTAGAGTC pLKO.1 890 CDS 100% 4.050 2.025 Y CHKB n/a
10 TRCN0000199248 CTGGTAGAAGTCAGTCGGTAT pLKO.1 1233 CDS 100% 4.050 2.025 Y CHKB n/a
11 TRCN0000052556 GAAAGCGTGATGTTCGCCATA pLKO.1 573 CDS 100% 4.050 2.025 Y CHKB n/a
12 TRCN0000199156 CGCAATTTCATGGCATGGAGA pLKO.1 733 CDS 100% 2.640 1.320 Y CHKB n/a
13 TRCN0000199501 GCAGTCTAAGTGCCCGGACAC pLKO.1 290 CDS 100% 0.000 0.000 Y CHKB n/a
14 TRCN0000024965 GAAAGCGTGATGTTCGCCATT pLKO.1 573 CDS 100% 4.050 2.025 Y Chkb n/a
15 TRCN0000274564 GAAAGCGTGATGTTCGCCATT pLKO_005 573 CDS 100% 4.050 2.025 Y Chkb n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005198.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489576 GCGTTCTACATATGACCCTTATTA pLX_317 28.3% 100% 100% V5 (not translated due to prior stop codon) n/a
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