Construct: ORF TRCN0000489576
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020338.1_s317c1
- DNA Barcode:
- GCGTTCTACATATGACCCTTATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CHKB (1120)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489576
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1120 | CHKB | choline kinase beta | NM_005198.4 | 100% | 100% | |
| 2 | human | 386593 | CHKB-CPT1B | CHKB-CPT1B readthrough (NMD... | NR_027928.2 | 27.6% | 1_218del;1404_4293del | |
| 3 | mouse | 12651 | Chkb | choline kinase beta | NM_007692.6 | 85.2% | 85.8% | (many diffs) |
| 4 | mouse | 12651 | Chkb | choline kinase beta | NR_037154.1 | 55.9% | (many diffs) | |
| 5 | mouse | 12651 | Chkb | choline kinase beta | NR_037153.1 | 52% | (many diffs) | |
| 6 | mouse | 100134861 | Gm44502 | predicted readthrough trans... | NR_004843.2 | 22.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1257
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcggcc gaggcgacag ctgtggccgg aagcggggct gttggcggct 121 gcctggccaa agacggcttg cagcagtcta agtgcccgga cactacccca aaacggcggc 181 gcgcctcgtc gctgtcgcgt gacgccgagc gccgagccta ccaatggtgc cgggagtact 241 tgggcggggc ctggcgccga gtgcagcccg aggagctgag ggtttacccc gtgagcggag 301 gcctcagcaa cctgctcttc cgctgctcgc tcccggacca cctgcccagc gttggcgagg 361 agccccggga ggtgcttctg cggctgtacg gagccatctt gcagggcgtg gactccctgg 421 tgctagaaag cgtgatgttc gccatacttg cggagcggtc gctggggccc cagctgtacg 481 gagtcttccc agagggccgg ctggaacagt acatcccaag tcggccattg aaaactcaag 541 agcttcgaga gccagtgttg tcagcagcca ttgccacgaa gatggcgcaa tttcatggca 601 tggagatgcc tttcaccaag gagccccact ggctgtttgg gaccatggag cggtacctaa 661 aacagatcca ggacctgccc ccaactggcc tccctgagat gaacctgctg gagatgtaca 721 gcctgaagga tgagatgggc aacctcagga agttactaga gtctacccca tcgccagtcg 781 tcttctgcca caatgacatc caggaaggga acatcttgct gctctcagag ccagaaaatg 841 ctgacagcct catgctggtg gacttcgagt acagcagtta taactatagg ggctttgaca 901 ttgggaacca tttttgtgag tgggttTATG ATTATACTCA CGAGGAATGG CCTTTCTACA 961 AAGCAAGGCC CACAGACTAC CCCACTCAAG AACAGCAGTT GCATTTTATT CGTCATTACC 1021 TGGCAGAGGC AAAGAAAGGT GAGACCCTCT CCCAAGAGGA GCAGAGAAAA CTGGAAGAAG 1081 ATTTGCTGGT AGAAGTCAGT CGGTATGCTC TGGCATCCCA TTTCTTCTGG GGTCTGTGGT 1141 CCATCCTCCA GGCATCCATG TCCACCATAG AATTTGGTTA CTTGGACTAT GCCCAGTCTC 1201 GGTTCCAGTT CTACTTCCAG CAGAAGGGGC AGCTGACCAG TGTCCACTCC TCATCCTGAG 1261 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1321 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1381 TTTATATATC TTGTGGAAAG GACGAGCGTT CTACATATGA CCCTTATTAA CGCGTTAAGT 1441 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt