Transcript: Human NM_005626.5

Homo sapiens serine and arginine rich splicing factor 4 (SRSF4), mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
SRSF4 (6429)
Length:
2300
CDS:
149..1633

Additional Resources:

NCBI RefSeq record:
NM_005626.5
NBCI Gene record:
SRSF4 (6429)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005626.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000006626 CGCACAGAGTACAGACTTATT pLKO.1 449 CDS 100% 13.200 18.480 N SRSF4 n/a
2 TRCN0000231449 GACGCAGTGGATATGGTTATA pLKO_005 393 CDS 100% 13.200 18.480 N SRSF4 n/a
3 TRCN0000231448 CTTTGTGGTGAGCGAGTAATT pLKO_005 326 CDS 100% 13.200 10.560 N SRSF4 n/a
4 TRCN0000006625 GCACAAGTGATTGGAGTAGAA pLKO.1 1694 3UTR 100% 4.950 3.960 N SRSF4 n/a
5 TRCN0000006628 GCTCCAGATCAAAGTCAGCTT pLKO.1 1524 CDS 100% 2.640 2.112 N SRSF4 n/a
6 TRCN0000231451 CCCTAATGGTGTGTCTATAAT pLKO_005 1742 3UTR 100% 15.000 10.500 N SRSF4 n/a
7 TRCN0000231450 CAAGACCTAAAGGATTATATG pLKO_005 500 CDS 100% 13.200 9.240 N SRSF4 n/a
8 TRCN0000231447 GTGGATCTGAAGAACGGATAT pLKO_005 239 CDS 100% 10.800 7.560 N SRSF4 n/a
9 TRCN0000006629 GCAGTGGATATGGTTATAGAA pLKO.1 396 CDS 100% 5.625 3.938 N SRSF4 n/a
10 TRCN0000006627 GCAGTCATTCTAAGAGTAGAT pLKO.1 786 CDS 100% 4.950 3.465 N SRSF4 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005626.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01523 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01523 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000481291 ACGCTTAGAATAAATTACATATGA pLX_317 31.7% 100% 100% V5 n/a
Download CSV