Construct: ORF TRCN0000481291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002293.1_s317c1
- Derived from:
- ccsbBroadEn_01523
- DNA Barcode:
- ACGCTTAGAATAAATTACATATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRSF4 (6429)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6429 | SRSF4 | serine and arginine rich sp... | NM_005626.5 | 100% | 100% | |
| 2 | human | 6429 | SRSF4 | serine and arginine rich sp... | XM_011541951.3 | 93.9% | 93.9% | 576_577ins90 |
| 3 | mouse | 57317 | Srsf4 | serine/arginine-rich splici... | NM_020587.2 | 84.2% | 87.6% | (many diffs) |
| 4 | mouse | 57317 | Srsf4 | serine/arginine-rich splici... | XM_017320335.1 | 61.2% | 63% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1548
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gcgggtgtac atcggccgcc tgagctacca ggcccgggag cgcgatgtgg 121 agcgcttctt taagggctac gggaagatcc tggaggtgga tctgaagaac ggatatggtt 181 ttgtggagtt tgatgatctg cgtgatgcag atgatgctgt ttatgaactg aatggcaaag 241 acctttgtgg tgagcgagta attgttgagc atgcccgcgg cccacggcga gatggcagtt 301 acggttctgg acgcagtgga tatggttata gaagaagtgg ccgagataaa tatggccctc 361 ctactcgcac agagtacaga cttattgtgg agaatttgtc aagtcggtgc agctggcaag 421 acctaaagga ttatatgcgt caggcaggag aagtgactta tgcagatgct cacaagggac 481 gcaaaaatga aggggtgatt gaatttgtat cttattctga tatgaaaaga gctttggaaa 541 agttggatgg aactgaagtc aatgggagaa aaatcagatt agttgaagac aagccaggtt 601 ccagacgacg ccggtcctac tccagaagcc ggagtcattc aaggtctcgc tctcgaagca 661 gacattcccg taagagcaga agccgaagtg gcagcagcaa aagcagtcat tctaagagta 721 gatctcggtc caggtcgggc tcccgctccc ggagcaagag ccggagccgg agccagagtc 781 ggagccggag caagaaagag aaaagcagga gccccagcaa ggaaaagagc cgcagccgca 841 gccatagcgc tggcaagagc cgcagcaaga gcaaagacca agctgaagag aagatccaaa 901 acaatgacaa tgtcgggaaa cccaagagcc ggagtcctag caggcataaa agtaagagca 961 aaagtcggag caggagtcag gagaggagag tggaggagga gaagcgaggg agtgtgagca 1021 ggggcaggag ccaggagaag agcctccgcc agagtcggag ccggagcagg agcaaagggg 1081 gCAGCAGGAG CCGGAGCAGG AGCCGCAGCA AGAGCAAGGA CAAGAGGAAG GGCAGGAAGA 1141 GAAGCAGAGA GGAGAGCCGC AGTCGCAGTC GCAGCCGCAG CAAGAGTGAG AGGAGCAGAA 1201 AGCGAGGCAG CAAGCGAGAC AGCAAGGCGG GCAGCAGCAA GAAGAAGAAG AAGGAAGACA 1261 CTGACCGCTC CCAGTCCAGA TCTCCATCCC GCTCCGTGTC AAAGGAGCGG GAACATGCCA 1321 AGTCTGAATC CAGCCAGAGG GAAGGTCGAG GAGAGAGTGA GAATGCTGGC ACCAATCAGG 1381 AGACCCGGTC CAGGTCGAGA TCCAATTCCA AATCGAAACC AAACCTTCCA TCAGAATCAC 1441 GCTCCAGATC AAAGTCAGCT TCAAAAACCC GATCTCGGTC CAAGTCTAGA TCCAGGTCTG 1501 CTTCCAGATC GCCCTCCCGA TCTAGATCTA GGTCCCACTC AAGGTCCTAC CCAACTTTCT 1561 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1621 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1681 GTGGAAAGGA CGAACGCTTA GAATAAATTA CATATGAACG CGTTAAGTCg acaatcaacc 1741 tctggattac aaaatttgtg aaagatt