Transcript: Mouse NM_008801.2

Mus musculus phosphodiesterase 6D, cGMP-specific, rod, delta (Pde6d), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Pde6d (18582)
Length:
1109
CDS:
153..605

Additional Resources:

NCBI RefSeq record:
NM_008801.2
NBCI Gene record:
Pde6d (18582)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008801.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000114885 GCTTCAAACTAAATTGGATGA pLKO.1 193 CDS 100% 4.050 3.240 N Pde6d n/a
2 TRCN0000332322 GCTTCAAACTAAATTGGATGA pLKO_005 193 CDS 100% 4.050 3.240 N Pde6d n/a
3 TRCN0000114884 TGTCATCATAGAGACAAAGTT pLKO.1 530 CDS 100% 5.625 3.938 N Pde6d n/a
4 TRCN0000332395 TGTCATCATAGAGACAAAGTT pLKO_005 530 CDS 100% 5.625 3.938 N Pde6d n/a
5 TRCN0000114882 CAATGTCATCATAGAGACAAA pLKO.1 527 CDS 100% 4.950 3.465 N Pde6d n/a
6 TRCN0000114881 GCTTTCTTTAGCCTCTGGTTT pLKO.1 924 3UTR 100% 4.950 3.465 N Pde6d n/a
7 TRCN0000354139 GCTTTCTTTAGCCTCTGGTTT pLKO_005 924 3UTR 100% 4.950 3.465 N Pde6d n/a
8 TRCN0000114883 GAAGAGTGGTTCTTCGAGTTT pLKO.1 414 CDS 100% 0.495 0.347 N Pde6d n/a
9 TRCN0000332394 GAAGAGTGGTTCTTCGAGTTT pLKO_005 414 CDS 100% 0.495 0.347 N Pde6d n/a
10 TRCN0000048851 ACAGGGAAGATACTCTGGCAA pLKO.1 231 CDS 100% 2.640 1.584 N PDE6D n/a
11 TRCN0000048850 GTGATCCCTAACTCCACAAAT pLKO.1 441 CDS 100% 13.200 7.920 N PDE6D n/a
12 TRCN0000290635 GTGATCCCTAACTCCACAAAT pLKO_005 441 CDS 100% 13.200 7.920 N PDE6D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008801.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01158 pDONR223 100% 93.3% 98% None (many diffs) n/a
2 ccsbBroad304_01158 pLX_304 0% 93.3% 98% V5 (many diffs) n/a
3 TRCN0000468394 TGCAGCTTTACAACATGCACTCTG pLX_317 64.6% 93.3% 98% V5 (many diffs) n/a
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