Construct: ORF TRCN0000468394
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013778.1_s317c1
- Derived from:
- ccsbBroadEn_01158
- DNA Barcode:
- TGCAGCTTTACAACATGCACTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE6D (5147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468394
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5147 | PDE6D | phosphodiesterase 6D | NM_002601.4 | 100% | 100% | |
| 2 | human | 5147 | PDE6D | phosphodiesterase 6D | XM_011511342.1 | 87.3% | 87.3% | 0_1ins57 |
| 3 | human | 5147 | PDE6D | phosphodiesterase 6D | NM_001291018.1 | 62% | 58.6% | 265_266ins106;279_280ins65 |
| 4 | mouse | 18582 | Pde6d | phosphodiesterase 6D, cGMP-... | NM_008801.2 | 93.3% | 98% | (many diffs) |
| 5 | mouse | 18582 | Pde6d | phosphodiesterase 6D, cGMP-... | XM_011247941.2 | 57.4% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agccaaggac gagcgggcca gggagatcct gaggggcttc aaactaaatt 121 ggatgaacct tcgggatgct gagacaggga agatactctg gcaaggaaca gaagacctgt 181 ctgtccctgg tgtggagcat gaagcccgtg ttcccaagaa aaTCCTCAAG TGCAAGGCAG 241 TGTCTCGAGA ACTTAATTTT TCTTCGACAG AACAAATGGA AAAATTCCGC CTGGAACAAA 301 AAGTTTACTT CAAAGGGCAA TGCCTAGAAG AATGGTTCTT CGAGTTTGGC TTTGTGATCC 361 CTAACTCCAC AAATACCTGG CAGTCCTTGA TAGAGGCAGC ACCCGAGTCC CAGATGATGC 421 CAGCAAGCGT CTTAACTGGG AACGTTATCA TAGAAACAAA GTTTTTTGAC GACGATCTTC 481 TTGTAAGCAC ATCCAGAGTG AGACTTTTCT ATGTTTGCCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 ATGCAGCTTT ACAACATGCA CTCTGACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt