Transcript: Mouse NM_010240.2

Mus musculus ferritin light polypeptide 1 (Ftl1), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Ftl1 (14325)
Length:
986
CDS:
256..807

Additional Resources:

NCBI RefSeq record:
NM_010240.2
NBCI Gene record:
Ftl1 (14325)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010240.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000444132 TCAAGTCTTGGACCAAGTAAA pLKO_005 928 3UTR 100% 13.200 6.600 Y Ftl2-ps n/a
2 TRCN0000250445 ATCTGGATAAGGAGGTGAAAC pLKO_005 656 CDS 100% 10.800 5.400 Y Ftl1 n/a
3 TRCN0000250444 CCATGGAGAAGAACCTGAATC pLKO_005 560 CDS 100% 10.800 5.400 Y Ftl1 n/a
4 TRCN0000250443 CTCTGGGCGAGTATCTCTTTG pLKO_005 761 CDS 100% 10.800 5.400 Y Ftl1 n/a
5 TRCN0000265299 GAAGCCATCTCAAGATGAATG pLKO_005 504 CDS 100% 10.800 5.400 Y Ftl1 n/a
6 TRCN0000439474 AGGTGAAACTCATCAAGAAGA pLKO_005 668 CDS 100% 4.950 2.475 Y Ftl2-ps n/a
7 TRCN0000196221 GATGTGCAGAAGCCATCTCAA pLKO.1 496 CDS 100% 4.950 2.475 Y Ftl2-ps n/a
8 TRCN0000451671 CCTCACTCTCAAGCACGACTA pLKO_005 786 CDS 100% 4.050 2.025 Y Ftl2-ps n/a
9 TRCN0000444414 GAACCTGAATCAGGCCCTCTT pLKO_005 570 CDS 100% 4.050 2.025 Y Ftl2-ps n/a
10 TRCN0000453299 CACTCTTCCAGGATGTGCAGA pLKO_005 485 CDS 100% 2.640 1.320 Y Ftl2-ps n/a
11 TRCN0000449628 TATCTCTTTGAGCGCCTCACT pLKO_005 772 CDS 100% 2.640 1.320 Y Ftl2-ps n/a
12 TRCN0000258043 TACCTCTCTCTGGGCTTCTTT pLKO_005 346 CDS 100% 0.000 0.000 Y Ftl1 n/a
13 TRCN0000450217 TGACTTCCTGGAAAGCCACTT pLKO_005 636 CDS 100% 4.050 2.025 Y Ftl2-ps n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010240.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00594 pDONR223 100% 81.9% 78.1% None (many diffs) n/a
2 ccsbBroad304_00594 pLX_304 0% 81.9% 78.1% V5 (many diffs) n/a
3 TRCN0000471307 CGGGGGCATCATTGAAGAAAGTCA pLX_317 80% 81.9% 78.1% V5 (many diffs) n/a
4 TRCN0000470657 CTTACCGTACTACTGTAATCAAAC pLX_317 84.9% 81.9% 78.1% V5 (many diffs) n/a
5 ccsbBroadEn_06226 pDONR223 100% 81.6% 77.5% None (many diffs) n/a
6 ccsbBroad304_06226 pLX_304 0% 81.6% 77.5% V5 (many diffs) n/a
7 ccsbBroadEn_06227 pDONR223 100% 81.7% 77.5% None (many diffs) n/a
8 ccsbBroad304_06227 pLX_304 0% 81.7% 77.5% V5 (many diffs) n/a
9 ccsbBroadEn_15418 pDONR223 0% 81.7% 78.1% None (many diffs) n/a
10 ccsbBroad304_15418 pLX_304 0% 81.7% 78.1% V5 (many diffs) n/a
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