Construct: ORF TRCN0000471307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004962.1_s317c1
- Derived from:
- ccsbBroadEn_00594
- DNA Barcode:
- CGGGGGCATCATTGAAGAAAGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FTL (2512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2512 | FTL | ferritin light chain | NM_000146.4 | 100% | 100% | |
| 2 | human | 2512 | FTL | ferritin light chain | XM_024451447.1 | 50.7% | 50.7% | 1_510del |
| 3 | mouse | 14325 | Ftl1 | ferritin light polypeptide 1 | NM_010240.2 | 81.9% | 78.1% | (many diffs) |
| 4 | mouse | 100862446 | LOC100862446 | ferritin light chain 1 | XM_003689208.3 | 81.6% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcccagatt cgtcagaatt attccaccga cgtggaggca gccgtcaaca 121 gcctggtcaa tttgtacctg caggcctcct acacctacct ctctcTGGGC TTCTATTTCG 181 ACCGCGATGA TGTGGCTCTG GAAGGCGTGA GCCACTTCTT CCGCGAATTG GCCGAGGAGA 241 AGCGCGAGGG CTACGAGCGT CTCCTGAAGA TGCAAAACCA GCGTGGCGGC CGCGCTCTCT 301 TCCAGGACAT CAAGAAGCCA GCTGAAGATG AGTGGGGTAA AACCCCAGAC GCCATGAAAG 361 CTGCCATGGC CCTGGAGAAA AAGCTGAACC AGGCCCTTTT GGATCTTCAT GCCCTGGGTT 421 CTGCCCGCAC GGACCCCCAT CTCTGTGACT TCCTGGAGAC TCACTTCCTA GATGAGGAAG 481 TGAAGCTTAT CAAGAAGATG GGTGACCACC TGACCAACCT CCACAGGCTG GGTGGCCCGG 541 AGGCTGGGCT GGGCGAGTAT CTCTTCGAAA GGCTCACTCT CAAGCACGAC TACCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGACGGG GGCATCATTG AAGAAAGTCA ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt