Transcript: Mouse NM_010302.2

Mus musculus guanine nucleotide binding protein, alpha 12 (Gna12), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Gna12 (14673)
Length:
1880
CDS:
136..1275

Additional Resources:

NCBI RefSeq record:
NM_010302.2
NBCI Gene record:
Gna12 (14673)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_010302.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000097861 CGTGGAACATGACTTCGTTAT pLKO.1 762 CDS 100% 10.800 15.120 N Gna12 n/a
2 TRCN0000097863 CGAGACCATCGTCAACAACAA pLKO.1 960 CDS 100% 4.950 6.930 N Gna12 n/a
3 TRCN0000097860 CGCCTCTCTACAGAAACTCTT pLKO.1 1553 3UTR 100% 4.950 6.930 N Gna12 n/a
4 TRCN0000097864 CTGGATAACTTGGACCGGATT pLKO.1 676 CDS 100% 4.050 5.670 N Gna12 n/a
5 TRCN0000417545 ACGAGAAGCACGGGATGTTTC pLKO_005 500 CDS 100% 10.800 8.640 N Gna12 n/a
6 TRCN0000419109 ATCTTCGACAACATCCTTAAG pLKO_005 418 CDS 100% 10.800 7.560 N Gna12 n/a
7 TRCN0000036757 CGTCAACAACAAGCTCTTCTT pLKO.1 969 CDS 100% 4.950 3.465 N GNA12 n/a
8 TRCN0000097862 GCTGGGTGAATCAGTGAAGTA pLKO.1 651 CDS 100% 4.950 3.465 N Gna12 n/a
9 TRCN0000437691 AGAACATCCGCTTCGTGTTTC pLKO_005 1199 CDS 100% 10.800 6.480 N Gna12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_010302.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489771 ACATACATTAATACTCTCTAACTT pLX_317 18.6% 91.8% 98.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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