Construct: ORF TRCN0000489771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021887.1_s317c1
- DNA Barcode:
- ACATACATTAATACTCTCTAACTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GNA12 (2768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489771
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2768 | GNA12 | G protein subunit alpha 12 | NM_007353.3 | 99.9% | 100% | 120C>T |
| 2 | human | 2768 | GNA12 | G protein subunit alpha 12 | NM_001293092.2 | 95.4% | 95.5% | 120C>T;519_520ins51 |
| 3 | human | 2768 | GNA12 | G protein subunit alpha 12 | NM_001282441.2 | 77.5% | 74.1% | (many diffs) |
| 4 | human | 2768 | GNA12 | G protein subunit alpha 12 | NM_001282440.1 | 67.7% | 58.5% | (many diffs) |
| 5 | mouse | 14673 | Gna12 | guanine nucleotide binding ... | NM_010302.2 | 91.8% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1212
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtccggggtg gtgcggaccc tcagccgctg cctgctgccg gccgaggccg 121 gcggggcccg cgagcgcagg gcgggcagcg gcgcgcgcga cgcggagcgc gaggcccgga 181 ggcgtagtcg cgacatcgac gcgctgctgg cccgcgagcg gcgcgcggtc cggcgcctgg 241 tgaagatcct gctgctgggc gcgggcgaga gcggcaagtc cacgttcctc aagcagatgc 301 gcatcatcca cggccgcgag ttcgaccaga aggcgctgct ggagttccgc gacaccatct 361 tcgacaacat cctcaagggc tcaagggttc ttgttgatgc acgagataag cttggcattc 421 cttggcagta ttctgaaaat gagaagcatg ggatgttcct gatggccttc gagaacaagg 481 cggggctgcc tgtggagccg gccaccttcc agctgtacgt cccggccctg agcgcactct 541 ggagggattc tggcatcagg gaggctttca gccggagaag cgagtttcag ctgggggagt 601 cggtgaagta cttcctggac aacttggacc ggatcggcca gctgaattac tttcctagta 661 agcaagatat cctgctggct aggaaagcca ccaagggaat tgtggagcat gacttcgtta 721 ttaagaagat cccctttaag atggtggatg tgggcggcca gcggtcccag cgccagaagt 781 ggttccagtg cttcgacggg atcacgtcca tcctgttcat ggtctcctcc agcgagtacg 841 accaggtcct catggaggac aggcgcacca accggctggt ggagtccatg aacatcttcg 901 agaccatcgt caacaacaag ctcttcttca acgtctccat cattctcttc ctcaacaaga 961 tggacctcct ggtggagaag gtgaagaccg tgagcatcaa gaagcacTTC CCGGACTTCA 1021 GGGGCGACCC GCACAGGCTG GAGGACGTCC AGCGCTACCT GGTCCAGTGC TTCGACAGGA 1081 AGAGACGGAA CCGCAGCAAG CCACTCTTCC ACCACTTCAC CACCGCCATC GACACCGAGA 1141 ACGTCCGCTT CGTGTTCCAT GCTGTGAAAG ACACCATCCT GCAGGAGAAC CTGAAGGACA 1201 TCATGCTGCA GTAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1261 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1321 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACATACATTA ATACTCTCTA 1381 ACTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt