Transcript: Human NM_013436.5

Homo sapiens NCK associated protein 1 (NCKAP1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
NCKAP1 (10787)
Length:
20332
CDS:
359..3745

Additional Resources:

NCBI RefSeq record:
NM_013436.5
NBCI Gene record:
NCKAP1 (10787)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_013436.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000155763 CCGACACTATGCCTTGTGAAT pLKO.1 1092 CDS 100% 4.950 6.930 N NCKAP1 n/a
2 TRCN0000319139 CCGACACTATGCCTTGTGAAT pLKO_005 1092 CDS 100% 4.950 6.930 N NCKAP1 n/a
3 TRCN0000152104 CTTAAAGAATTTCTGGCGCTT pLKO.1 3521 CDS 100% 2.160 3.024 N NCKAP1 n/a
4 TRCN0000151941 CCTCTCAATCAAGATACTCAA pLKO.1 2037 CDS 100% 4.950 3.960 N NCKAP1 n/a
5 TRCN0000319140 CCTCTCAATCAAGATACTCAA pLKO_005 2037 CDS 100% 4.950 3.960 N NCKAP1 n/a
6 TRCN0000154905 GCACAGAGAAAGACGCAAGTT pLKO.1 1372 CDS 100% 4.950 3.465 N NCKAP1 n/a
7 TRCN0000349668 GCACAGAGAAAGACGCAAGTT pLKO_005 1372 CDS 100% 4.950 3.465 N NCKAP1 n/a
8 TRCN0000155526 GCAGAGGTATTACGTGCAGTA pLKO.1 1636 CDS 100% 4.050 2.835 N NCKAP1 n/a
9 TRCN0000319212 GCAGAGGTATTACGTGCAGTA pLKO_005 1636 CDS 100% 4.050 2.835 N NCKAP1 n/a
10 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 9593 3UTR 100% 4.950 2.475 Y ORAI2 n/a
11 TRCN0000014248 CCCACTTACAAGTGAGAACAT pLKO.1 10825 3UTR 100% 4.950 2.475 Y RGR n/a
12 TRCN0000074123 CGCCTGTAATCCCAACACTTT pLKO.1 9193 3UTR 100% 4.950 2.475 Y GJD4 n/a
13 TRCN0000166650 CGCCTGTAATCCCAACACTTT pLKO.1 9193 3UTR 100% 4.950 2.475 Y C9orf85 n/a
14 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 5933 3UTR 100% 4.050 2.025 Y P3H4 n/a
15 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 5933 3UTR 100% 4.050 2.025 Y ORAI2 n/a
16 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 5933 3UTR 100% 4.050 2.025 Y P3H4 n/a
17 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6674 3UTR 100% 5.625 2.813 Y KLHL30 n/a
18 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 9590 3UTR 100% 4.950 2.475 Y LOC339059 n/a
19 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6674 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013436.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.