Transcript: Mouse NM_013540.2

Mus musculus glutamate receptor, ionotropic, AMPA2 (alpha 2) (Gria2), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Gria2 (14800)
Length:
6841
CDS:
408..3059

Additional Resources:

NCBI RefSeq record:
NM_013540.2
NBCI Gene record:
Gria2 (14800)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_013540.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000102940 CGTGTTATGACTCCAGAATTT pLKO.1 3132 3UTR 100% 13.200 18.480 N Gria2 n/a
2 TRCN0000061683 CCAGGTTATTACCATTGGAAA pLKO.1 1070 CDS 100% 4.950 6.930 N GRIA2 n/a
3 TRCN0000102941 CCAATGGGATAAGTTCGCATA pLKO.1 836 CDS 100% 4.050 5.670 N Gria2 n/a
4 TRCN0000426386 TGCGGCAAGGATGCGATATTT pLKO_005 2224 CDS 100% 15.000 19.500 N GRIA2 n/a
5 TRCN0000102944 CCAAACATTGTGGATTCAAGT pLKO.1 1768 CDS 100% 4.950 3.960 N Gria2 n/a
6 TRCN0000102942 GCCATTCATGAGCCTTGGAAT pLKO.1 1949 CDS 100% 4.950 3.465 N Gria2 n/a
7 TRCN0000102943 CCTCGCAGAATTCCCAGAATT pLKO.1 2983 CDS 100% 0.000 0.000 N Gria2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013540.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15434 pDONR223 0% 78.9% 84.2% None (many diffs) n/a
2 ccsbBroad304_15434 pLX_304 0% 78.9% 84.2% V5 (many diffs) n/a
3 TRCN0000466239 GCACCCCATCATGTAGGAATTACG pLX_317 14.1% 78.9% 84.2% V5 (many diffs) n/a
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