Construct: ORF TRCN0000466239

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017926.1_s317c1
Derived from:
ccsbBroadEn_15434
DNA Barcode:
GCACCCCATCATGTAGGAATTACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRIA2 (2891)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466239
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2891 GRIA2 glutamate ionotropic recept... NM_001083620.1 90.3% 90.3% (many diffs)
2 human 2891 GRIA2 glutamate ionotropic recept... NM_000826.4 85.4% 85.3% (many diffs)
3 human 2891 GRIA2 glutamate ionotropic recept... NM_001083619.1 84.1% 84.4% (many diffs)
4 human 2891 GRIA2 glutamate ionotropic recept... XR_001741212.2 21.8% (many diffs)
5 mouse 14800 Gria2 glutamate receptor, ionotro... NM_001083806.1 80.2% 85.2% (many diffs)
6 mouse 14800 Gria2 glutamate receptor, ionotro... NM_013540.2 78.9% 84.2% (many diffs)
7 mouse 14800 Gria2 glutamate receptor, ionotro... XM_006501012.3 76.3% 80.1% (many diffs)
8 mouse 14800 Gria2 glutamate receptor, ionotro... XM_006501013.3 75.1% 79.1% (many diffs)
9 mouse 14800 Gria2 glutamate receptor, ionotro... XM_006501015.3 70.3% 71.3% (many diffs)
10 mouse 14800 Gria2 glutamate receptor, ionotro... XM_006501014.2 69.8% 75% (many diffs)
11 mouse 14800 Gria2 glutamate receptor, ionotro... NM_001039195.1 66.6% 71.8% (many diffs)
12 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783658.1 51.7% (many diffs)
13 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783652.1 22.5% (many diffs)
14 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783661.1 22.5% (many diffs)
15 mouse 14800 Gria2 glutamate receptor, ionotro... XR_375489.3 22.4% (many diffs)
16 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783657.1 21.8% (many diffs)
17 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783654.1 21.8% (many diffs)
18 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783656.1 21.8% (many diffs)
19 mouse 14800 Gria2 glutamate receptor, ionotro... XR_375488.2 21.6% (many diffs)
20 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783660.1 21.2% (many diffs)
21 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783653.1 21% (many diffs)
22 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783655.1 21% (many diffs)
23 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783662.1 21% (many diffs)
24 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783659.1 20.7% (many diffs)
25 mouse 14800 Gria2 glutamate receptor, ionotro... XR_001783663.1 20.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2334
ORF length:
2268
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag acccgacctc aaaggagctc tccttagctt gattgaatac tatcaatggg
121acaagtttgc atacctctat gacagtgaca gaggcttatc aacactgcaa gctgtgctgg
181attctgctgc tgaaaagaaa tggcaagtga ctgctatcaa tgtgggaaac attaacaatg
241acaagaaaga tgagatgtac cgatcacttt ttcaagatct ggagttaaaa aaggaacggc
301gtgtaattct ggactgtgaa agggataaag taaacgacat tgtagaccag gttattacca
361ttggaaaaca cgttaaaggg taccactaca tcattgcaaa tctgggattt actgatggag
421acctattaaa aatccagttt ggaggtgcaa atgtctctgg atttcagata gtggactatg
481atgattcgtt ggtatctaaa tttatagaaa gatggtcaac actggaagaa aaagaatacc
541ctggagctca cacaacaaca attaagtata cttctgctct gacctatgat gccgttcaag
601tgatgactga agccttccgc aacctaagga agcaaagaat tgaaatctcc cgaaggggga
661atgcaggaga ctgtctggca aacccagcag tgccctgggg acaaggtgta gaaatagaaa
721gggccctcaa acaggttcag gttgaaggtc tctcaggaaa tataaagttt gaccagaatg
781gaaaaagaat aaactataca attaacatca tggagctcaa aactaatggg ccccggaaga
841ttggctactg gagtgaagtg gacaaaatgg ttgttaccct tactgagctc ccttctggaa
901atgacacctc tgggcttgag aataagattg ttgttgtcac cacaattttg gaatctccgt
961atgttatgat gaagaaaaat catgaaatgc ttgaaggcaa tgagcgctat gagggctact
1021gtgttgacct ggctgcagaa atcgccaaac attgtgggtt caagtacaag ttgacaattg
1081ttggtgatgg caagtatggg gccagggatg cagacacgaa aatttggaat gggatggttg
1141gagaacttgt atatgggaaa gctgatattg caattgctcc attaactatt acccttgtga
1201gagaagaggt gattgacttc tcaaagccct tcatgagcct cgggatatct atcatgatca
1261agaagcctca gaagtccaaa ccaggagtgt tttcctttct tgatccttta gcctatgaga
1321tctggatgtg cattgttttt gcctacattg gggtcagtgt agttttattc ctggtcagca
1381gatttagccc ctacgagtgg cacactgagg agtttgaaga tggaagagaa acacaaagta
1441gtgaatcaac taatgaattt gggattttta atagtctctg gttttccttg ggtgccttta
1501tgcggcaggg atgcgatatt tcgccaagat ccctctctgg gcgcattgtt ggaggtgtgt
1561ggtggttctt taccctgatc ataatctcct cctacacggc taacttagct gccttcctga
1621ctgtagagag gatggtgtct cccatcgaaa gtgctgagga tctttctaag caaacagaaa
1681ttgcttatgg aacattagac tctggctcca ctaaagagtt tttcaggaga tctaaaattg
1741cagtgtttga taaaatgtgg acctacatgc ggagtgcgga gccctctgtg tttgtgagga
1801ctacggccga aggggtggct agagtgcgga agtccaaagg gaaatatgcc tacttgttgg
1861agtccacgat gaacgagtac attgagcaaa ggaagccttg cgacaccatg aaagttggtg
1921gaaacctgga ttccaaaggc tatggcatcg caacacctaa aggatcctca ttaagaaccc
1981cagtaaatct tgcagtattg aaactcagtg agcaaggcgt cTTAGACAAG CTGAAAAACA
2041AATGGTGGTA CGATAAAGGT GAATGTGGAG CCAAGGACTC TGGAAGTAAG GAAAAGACCA
2101GTGCCCTCAG TCTGAGCAAC GTTGCTGGAG TATTCTACAT CCTTGTCGGG GGCCTTGGTT
2161TGGCAATGCT GGTGGCTTTG ATTGAGTTCT GTTACAAGTC AAGGGCCGAG GCGAAACGAA
2221TGAAGGTGGC AAAGAATGCA CAGAATATTA ACCCATCTTC CTCGCAGAAT TCACAGAATT
2281TTGCAACTTA TAAGGAAGGT TACAACGTAT ATGGCATCGA AAGTGTTAAA ATTTACCCAA
2341CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
2401ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
2461TATCTTGTGG AAAGGACGAG CACCCCATCA TGTAGGAATT ACGACGCGTT AAGTCgacaa
2521tcaacctctg gattacaaaa tttgtgaaag att

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