Construct: ORF TRCN0000466239
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017926.1_s317c1
- Derived from:
- ccsbBroadEn_15434
- DNA Barcode:
- GCACCCCATCATGTAGGAATTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRIA2 (2891)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466239
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2891 | GRIA2 | glutamate ionotropic recept... | NM_001083620.1 | 90.3% | 90.3% | (many diffs) |
| 2 | human | 2891 | GRIA2 | glutamate ionotropic recept... | NM_000826.4 | 85.4% | 85.3% | (many diffs) |
| 3 | human | 2891 | GRIA2 | glutamate ionotropic recept... | NM_001083619.1 | 84.1% | 84.4% | (many diffs) |
| 4 | human | 2891 | GRIA2 | glutamate ionotropic recept... | XR_001741212.2 | 21.8% | (many diffs) | |
| 5 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | NM_001083806.1 | 80.2% | 85.2% | (many diffs) |
| 6 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | NM_013540.2 | 78.9% | 84.2% | (many diffs) |
| 7 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XM_006501012.3 | 76.3% | 80.1% | (many diffs) |
| 8 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XM_006501013.3 | 75.1% | 79.1% | (many diffs) |
| 9 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XM_006501015.3 | 70.3% | 71.3% | (many diffs) |
| 10 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XM_006501014.2 | 69.8% | 75% | (many diffs) |
| 11 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | NM_001039195.1 | 66.6% | 71.8% | (many diffs) |
| 12 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783658.1 | 51.7% | (many diffs) | |
| 13 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783652.1 | 22.5% | (many diffs) | |
| 14 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783661.1 | 22.5% | (many diffs) | |
| 15 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_375489.3 | 22.4% | (many diffs) | |
| 16 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783657.1 | 21.8% | (many diffs) | |
| 17 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783654.1 | 21.8% | (many diffs) | |
| 18 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783656.1 | 21.8% | (many diffs) | |
| 19 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_375488.2 | 21.6% | (many diffs) | |
| 20 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783660.1 | 21.2% | (many diffs) | |
| 21 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783653.1 | 21% | (many diffs) | |
| 22 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783655.1 | 21% | (many diffs) | |
| 23 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783662.1 | 21% | (many diffs) | |
| 24 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783659.1 | 20.7% | (many diffs) | |
| 25 | mouse | 14800 | Gria2 | glutamate receptor, ionotro... | XR_001783663.1 | 20.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2334
- ORF length:
- 2268
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag acccgacctc aaaggagctc tccttagctt gattgaatac tatcaatggg 121 acaagtttgc atacctctat gacagtgaca gaggcttatc aacactgcaa gctgtgctgg 181 attctgctgc tgaaaagaaa tggcaagtga ctgctatcaa tgtgggaaac attaacaatg 241 acaagaaaga tgagatgtac cgatcacttt ttcaagatct ggagttaaaa aaggaacggc 301 gtgtaattct ggactgtgaa agggataaag taaacgacat tgtagaccag gttattacca 361 ttggaaaaca cgttaaaggg taccactaca tcattgcaaa tctgggattt actgatggag 421 acctattaaa aatccagttt ggaggtgcaa atgtctctgg atttcagata gtggactatg 481 atgattcgtt ggtatctaaa tttatagaaa gatggtcaac actggaagaa aaagaatacc 541 ctggagctca cacaacaaca attaagtata cttctgctct gacctatgat gccgttcaag 601 tgatgactga agccttccgc aacctaagga agcaaagaat tgaaatctcc cgaaggggga 661 atgcaggaga ctgtctggca aacccagcag tgccctgggg acaaggtgta gaaatagaaa 721 gggccctcaa acaggttcag gttgaaggtc tctcaggaaa tataaagttt gaccagaatg 781 gaaaaagaat aaactataca attaacatca tggagctcaa aactaatggg ccccggaaga 841 ttggctactg gagtgaagtg gacaaaatgg ttgttaccct tactgagctc ccttctggaa 901 atgacacctc tgggcttgag aataagattg ttgttgtcac cacaattttg gaatctccgt 961 atgttatgat gaagaaaaat catgaaatgc ttgaaggcaa tgagcgctat gagggctact 1021 gtgttgacct ggctgcagaa atcgccaaac attgtgggtt caagtacaag ttgacaattg 1081 ttggtgatgg caagtatggg gccagggatg cagacacgaa aatttggaat gggatggttg 1141 gagaacttgt atatgggaaa gctgatattg caattgctcc attaactatt acccttgtga 1201 gagaagaggt gattgacttc tcaaagccct tcatgagcct cgggatatct atcatgatca 1261 agaagcctca gaagtccaaa ccaggagtgt tttcctttct tgatccttta gcctatgaga 1321 tctggatgtg cattgttttt gcctacattg gggtcagtgt agttttattc ctggtcagca 1381 gatttagccc ctacgagtgg cacactgagg agtttgaaga tggaagagaa acacaaagta 1441 gtgaatcaac taatgaattt gggattttta atagtctctg gttttccttg ggtgccttta 1501 tgcggcaggg atgcgatatt tcgccaagat ccctctctgg gcgcattgtt ggaggtgtgt 1561 ggtggttctt taccctgatc ataatctcct cctacacggc taacttagct gccttcctga 1621 ctgtagagag gatggtgtct cccatcgaaa gtgctgagga tctttctaag caaacagaaa 1681 ttgcttatgg aacattagac tctggctcca ctaaagagtt tttcaggaga tctaaaattg 1741 cagtgtttga taaaatgtgg acctacatgc ggagtgcgga gccctctgtg tttgtgagga 1801 ctacggccga aggggtggct agagtgcgga agtccaaagg gaaatatgcc tacttgttgg 1861 agtccacgat gaacgagtac attgagcaaa ggaagccttg cgacaccatg aaagttggtg 1921 gaaacctgga ttccaaaggc tatggcatcg caacacctaa aggatcctca ttaagaaccc 1981 cagtaaatct tgcagtattg aaactcagtg agcaaggcgt cTTAGACAAG CTGAAAAACA 2041 AATGGTGGTA CGATAAAGGT GAATGTGGAG CCAAGGACTC TGGAAGTAAG GAAAAGACCA 2101 GTGCCCTCAG TCTGAGCAAC GTTGCTGGAG TATTCTACAT CCTTGTCGGG GGCCTTGGTT 2161 TGGCAATGCT GGTGGCTTTG ATTGAGTTCT GTTACAAGTC AAGGGCCGAG GCGAAACGAA 2221 TGAAGGTGGC AAAGAATGCA CAGAATATTA ACCCATCTTC CTCGCAGAAT TCACAGAATT 2281 TTGCAACTTA TAAGGAAGGT TACAACGTAT ATGGCATCGA AAGTGTTAAA ATTTACCCAA 2341 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2401 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2461 TATCTTGTGG AAAGGACGAG CACCCCATCA TGTAGGAATT ACGACGCGTT AAGTCgacaa 2521 tcaacctctg gattacaaaa tttgtgaaag att