Transcript: Mouse NM_017402.4

Mus musculus Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3, mRNA.

Source:
NCBI, updated 2016-09-15
Taxon:
Mus musculus (mouse)
Gene:
Arhgef7 (54126)
Length:
4488
CDS:
408..2348

Additional Resources:

NCBI RefSeq record:
NM_017402.4
NBCI Gene record:
Arhgef7 (54126)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_017402.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047595 CCTGGGATGAGACCAATCTAT pLKO.1 2326 CDS 100% 5.625 7.875 N ARHGEF7 n/a
2 TRCN0000110028 GCCCTCCCAAAGGGTTCGATA pLKO.1 643 CDS 100% 1.650 2.310 N Arhgef7 n/a
3 TRCN0000305874 GATACGGTGTATGCGTTAAAG pLKO_005 2181 CDS 100% 13.200 10.560 N Arhgef7 n/a
4 TRCN0000303860 AGAGACACATGGAGGATTATC pLKO_005 1135 CDS 100% 13.200 9.240 N ARHGEF7 n/a
5 TRCN0000305803 AGAGACACATGGAGGATTATC pLKO_005 1135 CDS 100% 13.200 9.240 N Arhgef7 n/a
6 TRCN0000110025 GCGAGAATAAAGCAATACAAA pLKO.1 3161 3UTR 100% 5.625 3.938 N Arhgef7 n/a
7 TRCN0000324967 GCGAGAATAAAGCAATACAAA pLKO_005 3161 3UTR 100% 5.625 3.938 N Arhgef7 n/a
8 TRCN0000110026 CCTGAAGGTTATCGAAGCTTA pLKO.1 2012 CDS 100% 4.950 3.465 N Arhgef7 n/a
9 TRCN0000110027 GAAGTCTATGACGGCCTTCAA pLKO.1 1178 CDS 100% 4.950 3.465 N Arhgef7 n/a
10 TRCN0000353879 GAAGTCTATGACGGCCTTCAA pLKO_005 1178 CDS 100% 4.950 3.465 N Arhgef7 n/a
11 TRCN0000110029 CAGATCCTGAAGGTTATCGAA pLKO.1 2007 CDS 100% 3.000 2.100 N Arhgef7 n/a
12 TRCN0000324966 CAGATCCTGAAGGTTATCGAA pLKO_005 2007 CDS 100% 3.000 2.100 N Arhgef7 n/a
13 TRCN0000047597 CTCTGCTACAAGGAGGATCTT pLKO.1 1872 CDS 100% 4.950 3.465 N ARHGEF7 n/a
14 TRCN0000112913 CAGGGCATCATGGGTTCTAAT pLKO.1 2893 3UTR 100% 13.200 6.600 Y Stom n/a
15 TRCN0000302745 CAGGGCATCATGGGTTCTAAT pLKO_005 2893 3UTR 100% 13.200 6.600 Y Stom n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_017402.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02036 pDONR223 100% 61.3% 63.7% None (many diffs) n/a
2 ccsbBroad304_02036 pLX_304 0% 61.3% 63.7% V5 (many diffs) n/a
3 TRCN0000479549 CAGCCCGAGTTGTATTTCACCCGA pLX_317 16% 61.3% 63.7% V5 (many diffs) n/a
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