Transcript: Human NM_017859.4

Homo sapiens uridine-cytidine kinase 1 like 1 (UCKL1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
UCKL1 (54963)
Length:
1838
CDS:
45..1691

Additional Resources:

NCBI RefSeq record:
NM_017859.4
NBCI Gene record:
UCKL1 (54963)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146668 AGCCGGAAGAAGGACTGGGT pXPR_003 AGG 581 35% 4 0.636 UCKL1 UCKL1 76345
2 BRDN0001147183 CCCAGGGAGCGGCAACACGG pXPR_003 TGG 856 52% 7 0.582 UCKL1 UCKL1 76347
3 BRDN0001162519 GTACCATCTACACCGCCGGG pXPR_003 CGG 246 15% 2 0.4867 UCKL1 UCKL1 76348
4 BRDN0001162217 TGTGGCCAGAATGATCATCG pXPR_003 AGG 355 22% 3 0.2803 UCKL1 UCKL1 76346
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_017859.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000037576 GCAGTACAACAAGTTTGTCAA pLKO.1 806 CDS 100% 4.950 6.930 N UCKL1 n/a
2 TRCN0000199954 GACCGCTACTTTGGGACAGAC pLKO.1 1623 CDS 100% 1.350 1.890 N UCKL1 n/a
3 TRCN0000199988 GCCTATGCATTTCCGCGAGTG pLKO.1 1527 CDS 100% 0.750 1.050 N UCKL1 n/a
4 TRCN0000381414 ACTTTGGCGACCGCTACTTTG pLKO_005 1615 CDS 100% 10.800 7.560 N UCKL1 n/a
5 TRCN0000322261 TCCTGGACATGAAGATCTTTG pLKO_005 700 CDS 100% 10.800 7.560 N Uckl1 n/a
6 TRCN0000037577 CGCACACAACAACTTCAACTT pLKO.1 485 CDS 100% 4.950 3.465 N UCKL1 n/a
7 TRCN0000037575 CCTCATCATTTCCACCCTCAA pLKO.1 533 CDS 100% 4.050 2.835 N UCKL1 n/a
8 TRCN0000037574 CGAGTTCATCTTCTACTCCAA pLKO.1 1088 CDS 100% 2.640 1.848 N UCKL1 n/a
9 TRCN0000197108 GCCCATTTATGACTTCACCAC pLKO.1 584 CDS 100% 2.160 1.512 N UCKL1 n/a
10 TRCN0000199191 CCCAAGGACATCAGCGATGAC pLKO.1 1365 CDS 100% 1.350 0.945 N UCKL1 n/a
11 TRCN0000037578 GCGGTGGACAAGCGGGTCAAT pLKO.1 1563 CDS 100% 0.000 0.000 N UCKL1 n/a
12 TRCN0000024877 CAGTACAACAAGTTTGTCAAA pLKO.1 807 CDS 100% 4.950 3.465 N Uckl1 n/a
13 TRCN0000322195 CAGTACAACAAGTTTGTCAAA pLKO_005 807 CDS 100% 4.950 3.465 N Uckl1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_017859.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489164 TCTGGCGGTCCCACGAATTCTCCA pLX_317 18.4% 99.7% 99.8% V5 (not translated due to prior stop codon) 1386C>A;1644_1645insTTG n/a
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