Construct: ORF TRCN0000489164
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020278.1_s317c1
- DNA Barcode:
- TCTGGCGGTCCCACGAATTCTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- UCKL1 (54963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489164
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_017859.4 | 99.7% | 99.8% | 1386C>A;1644_1645insTTG |
| 2 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353477.2 | 99.5% | 99.6% | 112_113insGCA;1383C>A;1641_1642insTTG |
| 3 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353475.2 | 98.9% | 98.9% | (many diffs) |
| 4 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_006723809.1 | 98.9% | 98.3% | (many diffs) |
| 5 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353476.2 | 98.7% | 98.7% | (many diffs) |
| 6 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_005260216.3 | 98.3% | 98.3% | 410_433del;1410C>A;1668_1669insTTG |
| 7 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_006723807.3 | 97.5% | 97.4% | (many diffs) |
| 8 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_006723806.3 | 97.4% | 96.9% | (many diffs) |
| 9 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001193379.2 | 95.4% | 94.1% | (many diffs) |
| 10 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353479.2 | 95.1% | 93.9% | (many diffs) |
| 11 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353478.2 | 94.3% | 93% | (many diffs) |
| 12 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451917.1 | 88.7% | 76.3% | (many diffs) |
| 13 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451918.1 | 88.5% | 76.1% | (many diffs) |
| 14 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148434.2 | 88.5% | (many diffs) | |
| 15 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148439.2 | 88.3% | (many diffs) | |
| 16 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_126526.2 | 88.2% | (many diffs) | |
| 17 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148441.2 | 87.9% | (many diffs) | |
| 18 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451915.1 | 87.6% | 75.3% | (many diffs) |
| 19 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451916.1 | 87.4% | 75.2% | (many diffs) |
| 20 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_244121.3 | 87.4% | (many diffs) | |
| 21 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_001754304.2 | 87.4% | (many diffs) | |
| 22 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451924.1 | 87.4% | 78.6% | (many diffs) |
| 23 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_001754302.2 | 87.2% | (many diffs) | |
| 24 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148436.2 | 87.1% | (many diffs) | |
| 25 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148435.2 | 87.1% | (many diffs) | |
| 26 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_430299.3 | 86.9% | (many diffs) | |
| 27 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_001754306.1 | 86.9% | (many diffs) | |
| 28 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451919.1 | 86.7% | 72.4% | (many diffs) |
| 29 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451925.1 | 86.5% | 77.7% | (many diffs) |
| 30 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451923.1 | 86.2% | 77.6% | (many diffs) |
| 31 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_936554.2 | 86.2% | (many diffs) | |
| 32 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_430301.3 | 86% | (many diffs) | |
| 33 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_001754305.2 | 86% | (many diffs) | |
| 34 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_001754303.2 | 85.8% | (many diffs) | |
| 35 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148442.2 | 85.7% | (many diffs) | |
| 36 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451922.1 | 85.6% | 76.8% | (many diffs) |
| 37 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451921.1 | 85.5% | 76.4% | (many diffs) |
| 38 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148437.2 | 85% | (many diffs) | |
| 39 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XR_244120.1 | 84.1% | (many diffs) | |
| 40 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148438.2 | 83.8% | (many diffs) | |
| 41 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451920.1 | 83.8% | 70.5% | (many diffs) |
| 42 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NR_148440.2 | 83.7% | (many diffs) | |
| 43 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353480.2 | 76.8% | 75.5% | (many diffs) |
| 44 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451931.1 | 76.8% | 75.5% | (many diffs) |
| 45 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451927.1 | 69.7% | 57.8% | (many diffs) |
| 46 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_017027897.1 | 69.3% | 52.1% | (many diffs) |
| 47 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451926.1 | 68.8% | 57% | (many diffs) |
| 48 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_017027895.2 | 68.4% | 51.5% | (many diffs) |
| 49 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_017027894.2 | 68.3% | 51.4% | (many diffs) |
| 50 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451928.1 | 68.2% | 55.1% | (many diffs) |
| 51 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451929.1 | 68.2% | 55.1% | (many diffs) |
| 52 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451930.1 | 68.2% | 55.1% | (many diffs) |
| 53 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353481.2 | 61.8% | 61.9% | 0_1ins624;762C>A;1020_1021insTTG |
| 54 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | NM_001353482.2 | 61.8% | 61.9% | 0_1ins624;762C>A;1020_1021insTTG |
| 55 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451932.1 | 54.6% | 43.1% | (many diffs) |
| 56 | human | 54963 | UCKL1 | uridine-cytidine kinase 1 l... | XM_024451933.1 | 52.5% | 41% | (many diffs) |
| 57 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | NM_026765.3 | 85.3% | 94.7% | (many diffs) |
| 58 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500705.2 | 83% | 91.4% | (many diffs) |
| 59 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500706.3 | 82.5% | 90.5% | (many diffs) |
| 60 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500704.2 | 82.4% | 90.5% | (many diffs) |
| 61 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500707.3 | 80.3% | 89.2% | (many diffs) |
| 62 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500708.3 | 80.1% | 89.2% | (many diffs) |
| 63 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500709.3 | 67.7% | 75.4% | (many diffs) |
| 64 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_006500710.3 | 67.7% | 75.4% | (many diffs) |
| 65 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_017319239.1 | 53.6% | 60.2% | (many diffs) |
| 66 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_017319240.1 | 51.4% | 57.9% | (many diffs) |
| 67 | mouse | 68556 | Uckl1 | uridine-cytidine kinase 1-l... | XM_011239960.2 | 50.9% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1719
- ORF length:
- 1647
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgcg cccccggccc gcgcggacgc tgatccttcg cccacgtcgc 121 cacctacggc ccgagacaca ccaggccggc aggctgagaa aagcgagacc gcgtgcgagg 181 accgcagcaa tgcagagtcc ctggacaggc tcctgccacc tgtgggcact gggcgctctc 241 cccggaagcg gaccaccagc cagtgcaagt cagagcctcc cctgctgcgt acaagcaagc 301 gtaccatcta caccgccggg cggccgccct ggtacaatga acacggcacg caatccaaag 361 aggccttcgc catcggcttg ggaggcggca gtgcctctgg gaagaccact gtggccagaa 421 tgatcatcga ggccctggat gtgccctggg tggtcttgct gtccatggac tccttctaca 481 aggtgctgac tgagcagcag caggaacagg ccgcacacaa caacttcaac ttcgaccacc 541 cagatgcctt tgacttcgac ctcatcattt ccaccctcaa gaagctgaag caggggaaga 601 gtgtcaaggt gcccatttat gacttcacca cgcacagccg gaagaaggac tggaaaacac 661 tgtatggtgc aaacgtcatc atctttgagg gcatcatggc ctttgctgac aagacactgt 721 tggagctcct ggacatgaag atctttgtgg acacagactc cgacatccgc ctggtacggc 781 ggctgcgccg ggacatcagt gagcgcggcc gggacatcga gggtgtcatc aagcagtaca 841 acaagtttgt caagccctcc ttcgaccagt acatccagcc caccatgcgc ctggcagaca 901 tcgtggtccc cagagggagc ggcaacacgg tggccatcga cctgattgtg cagcacgtgc 961 acagccagct ggaggagcgt gaactcagcg tcagggctgc gctggcctcg gcacaccagt 1021 gccacccgct gccccggacg ctgagcgtcc tgaagagcac gccgcaggta cggggcatgc 1081 acaccatcat cagggacaag gagaccagtc gcgacgagtt catcttctac tccaagagac 1141 tgatgcggct gctcatcgag cacgcgctct ccttcctgcc ctttcaggac tgcgtcgtac 1201 agaccccgca ggggcaggac tatgcgggca agtgctatgc ggggaagcag atcaccggtg 1261 tgtccattct gcgcgccggt gaaaccatgg agcccgcgct gcgcgctgtg tgcaaagacg 1321 tgcgcatcgg caccatcctc atccagacca accagcttac cggggagccc gagctccact 1381 acctgaggct gcccaaggac atcagcgatg accacgtgat ccTCATGGAC TGCACCGTGT 1441 CCACGGGCGC GGCGGCAATG ATGGCAGTGC GCGTGCTCCT GGACCACGAC GTGCCTGAGG 1501 ACAAGATCTT TTTGCTGTCG CTGCTCATGG CAGAGATGGG CGTGCACTCA GTGGCCTATG 1561 CATTTCCGCG AGTGAGAATC ATCACCACGG CGGTGGACAA GCGGGTCAAT GACCTTTTCC 1621 GCATCATCCC AGGCATTGGG AACTTTGGCG ACCGCTACTT TGGGACAGAC GCGGTCCCCG 1681 ATGGCAGTGA CGAGGAGGAA GTGGCCTACA CGGGTTTGTG AGACCCAGCT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TTCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGATCT GGCGGTCCCA CGAATTCTCC AACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t