Transcript: Human NM_021721.4

Homo sapiens ADAM metallopeptidase domain 22 (ADAM22), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
ADAM22 (53616)
Length:
2880
CDS:
324..2795

Additional Resources:

NCBI RefSeq record:
NM_021721.4
NBCI Gene record:
ADAM22 (53616)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021721.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232580 CTTCGTCGATATCCTCGTAAT pLKO_005 1002 CDS 100% 10.800 15.120 N ADAM22 n/a
2 TRCN0000052260 GCAGCTTATATTGGTGGGATT pLKO.1 1347 CDS 100% 4.050 5.670 N ADAM22 n/a
3 TRCN0000232582 ATGGGAGACACTGGCTATTAT pLKO_005 1530 CDS 100% 15.000 10.500 N ADAM22 n/a
4 TRCN0000232581 CCCAGTCATTAGCCCATAATA pLKO_005 1435 CDS 100% 15.000 10.500 N ADAM22 n/a
5 TRCN0000232583 TTAGTGCTGGCCCTCATATTA pLKO_005 2562 CDS 100% 15.000 10.500 N ADAM22 n/a
6 TRCN0000052259 GCAGTAAAGAAGGCACTATTT pLKO.1 2365 CDS 100% 13.200 9.240 N ADAM22 n/a
7 TRCN0000257370 GCAGTAAAGAAGGCACTATTT pLKO_005 2365 CDS 100% 13.200 9.240 N ADAM22 n/a
8 TRCN0000052258 GCCCAGTCATTAGCCCATAAT pLKO.1 1434 CDS 100% 13.200 9.240 N ADAM22 n/a
9 TRCN0000052262 CCAGAGATAGACAATGCAAAT pLKO.1 1969 CDS 100% 10.800 7.560 N ADAM22 n/a
10 TRCN0000052261 CCCTGACTCATTTGTTGCATT pLKO.1 755 CDS 100% 4.950 3.465 N ADAM22 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021721.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15859 pDONR223 0% 41.1% 39.8% None (many diffs) n/a
2 ccsbBroad304_15859 pLX_304 0% 41.1% 39.8% V5 (many diffs) n/a
3 TRCN0000473520 GGATTTATCCTGAAACACGATTGT pLX_317 40.8% 41.1% 39.8% V5 (many diffs) n/a
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