Construct: ORF TRCN0000473520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006202.1_s317c1
- Derived from:
- ccsbBroadEn_15859
- DNA Barcode:
- GGATTTATCCTGAAACACGATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM22 (53616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_021721.4 | 41.1% | 39.8% | (many diffs) |
| 2 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_004194.4 | 39.4% | 38.2% | (many diffs) |
| 3 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_016351.6 | 38.9% | 37.7% | (many diffs) |
| 4 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_001324417.2 | 38.8% | 37.6% | (many diffs) |
| 5 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012339.2 | 38.1% | 37% | (many diffs) |
| 6 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_001324421.2 | 38% | 36.9% | (many diffs) |
| 7 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_021722.6 | 37.6% | 36.5% | (many diffs) |
| 8 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_021723.5 | 37.3% | 36.2% | (many diffs) |
| 9 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012336.2 | 37.3% | 36.2% | (many diffs) |
| 10 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012337.2 | 37.3% | 36.2% | (many diffs) |
| 11 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012338.2 | 37.2% | 36.1% | (many diffs) |
| 12 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012335.2 | 36.9% | 35.8% | (many diffs) |
| 13 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012334.2 | 36.6% | 35.5% | (many diffs) |
| 14 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012333.2 | 36.5% | 35.4% | (many diffs) |
| 15 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_001324420.2 | 36.2% | 35.1% | (many diffs) |
| 16 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012332.2 | 36.2% | 35.1% | (many diffs) |
| 17 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516323.2 | 36.1% | 35.1% | (many diffs) |
| 18 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012331.2 | 36% | 34.9% | (many diffs) |
| 19 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012330.2 | 35.5% | 34.5% | (many diffs) |
| 20 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_017012329.2 | 35.5% | 34.4% | (many diffs) |
| 21 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_001324418.2 | 35.1% | 34% | (many diffs) |
| 22 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | NM_001324419.2 | 35% | 33.9% | (many diffs) |
| 23 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516322.2 | 34.8% | 33.8% | (many diffs) |
| 24 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_005250445.4 | 33.8% | 31.9% | (many diffs) |
| 25 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516318.2 | 33.8% | 32.8% | (many diffs) |
| 26 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516319.3 | 33.7% | 32.7% | (many diffs) |
| 27 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_006716029.4 | 33.1% | 31.3% | (many diffs) |
| 28 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516321.3 | 32.8% | 31% | (many diffs) |
| 29 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516320.3 | 32.6% | 30.7% | (many diffs) |
| 30 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_006716028.4 | 31.7% | 29.9% | (many diffs) |
| 31 | human | 53616 | ADAM22 | ADAM metallopeptidase domai... | XM_011516324.2 | 26.2% | 24.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggcggcagtg gctgtgtccg tgcccttctt gctgctctgt gtcctgggga 121 cctgccctcc ggcgcgctgc ggccaggcag gagacgcctc attgatggag ctagagaaga 181 ggaaggaaaa ccgcttcgtg gagcgccaga gcatcgtgcc actgcgcctc atctaccgct 241 cgggcggcga agacgaaagt cggcacgacg cgctcgacac gcgggtgcgg ggcgacgtcg 301 gtggcccgca gttgactcat gttgaccaag caagcttcca ggttgatgcc tttggaacgt 361 cattcattct cgatgtcgtg ctaaatcatg atttgctgtc ctctgaatac atagagagac 421 acattgaaca tggaggcaag actgtggaag ttaaaggagg agagcactgt tactaccagg 481 gccatatccg aggaaaccct gactcatttg ttgcattgtc aacatgccac ggacttcatg 541 ggatgttcta tgacgggaac cacacatatc tcattgagcc agaagaaaat gacactactc 601 aagaggattt ccattttcat tcagtttaca aatccagact gtttgaattt tccttggatg 661 atcttccatc tgaatTTCAG CAAGTAAACA TTACTCCATC AAAATTTATT TTGAAGCCAA 721 GACCAAAAAG GAGTAAACGG CAGCTTCGTC GATATCCTCG TAATGTAGAA GAAGAAACCA 781 AATACATTGA ACTGATGATT GTGAATGATC ACCTTATGTT TAAAAAACAT CGGCTTTCCG 841 TTGTACATAC CAATACCTAT GCGAAATCTG TGGTGAACAT GGCAGATTTA ATATATAAAG 901 ACCAACTTAA GACCAGGATA GTATTGGTTG CTATGGAAAC CTGGGCGACT GACAACAAGT 961 TTGCCATATC TGAAAATCCA TTGATCACCC TACGTGAGTT TATGAAATAC AGGAGGGATT 1021 TTATCAAAGA GAAAAGTGAT GCAGTTCACC TTTTTTCGTA CGTAACTTCT GTAATGATGT 1081 ATTACTTTTT TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGA TTTATCCTGA AACACGATTG 1261 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t